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Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

Journal Article


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Abstract


  • 3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated

    into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for

    Val and Leu, respectively. The extent of incorporation is up

    to B90%, and substituted protein is catalytically active. By

    contrast, 4-chloro-Val is not an effective replacement for Ile.

Authors


  •   Stigers, Dannon J. (external author)
  •   Watts, Zachary I. (external author)
  •   Hennessy, James E. (external author)
  •   Kim, Hye-Kyung (external author)
  •   Martini, Romeo (external author)
  •   Taylor, Matthew C. (external author)
  •   Ozawa, Kiyoshi (external author)
  •   Keillor, Jeffrey W. (external author)
  •   Dixon, Nicholas E.
  •   Prof Christopher Easton, Christopher (external author)

Publication Date


  • 2011

Citation


  • Stigers, D. J., Watts, Z. I., Hennessy, J. E., Kim, H., Martini, R., Taylor, M. C., Ozawa, K., Keillor, J. W., Dixon, N. E. & Easton, C. (2011). Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis. Chemical Communications, 47 (6), 1839-1841.

Scopus Eid


  • 2-s2.0-79251527551

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=8580&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5237

Has Global Citation Frequency


Number Of Pages


  • 2

Start Page


  • 1839

End Page


  • 1841

Volume


  • 47

Issue


  • 6

Abstract


  • 3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated

    into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for

    Val and Leu, respectively. The extent of incorporation is up

    to B90%, and substituted protein is catalytically active. By

    contrast, 4-chloro-Val is not an effective replacement for Ile.

Authors


  •   Stigers, Dannon J. (external author)
  •   Watts, Zachary I. (external author)
  •   Hennessy, James E. (external author)
  •   Kim, Hye-Kyung (external author)
  •   Martini, Romeo (external author)
  •   Taylor, Matthew C. (external author)
  •   Ozawa, Kiyoshi (external author)
  •   Keillor, Jeffrey W. (external author)
  •   Dixon, Nicholas E.
  •   Prof Christopher Easton, Christopher (external author)

Publication Date


  • 2011

Citation


  • Stigers, D. J., Watts, Z. I., Hennessy, J. E., Kim, H., Martini, R., Taylor, M. C., Ozawa, K., Keillor, J. W., Dixon, N. E. & Easton, C. (2011). Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis. Chemical Communications, 47 (6), 1839-1841.

Scopus Eid


  • 2-s2.0-79251527551

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=8580&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5237

Has Global Citation Frequency


Number Of Pages


  • 2

Start Page


  • 1839

End Page


  • 1841

Volume


  • 47

Issue


  • 6