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The effect of exercise on the skeletal muscle phospholipidome of rats fed a high-fat diet

Journal Article


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Abstract


  • The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague- Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in

    phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.

Authors


  •   Mitchell, Todd
  •   Turner, Nigel (external author)
  •   Else, Paul
  •   Hulbert, Tony J.
  •   Hawley, John A. (external author)
  •   Lee, Jong Sam (external author)
  •   Bruce, Clinton R. (external author)
  •   Blanksby, Stephen J. (external author)

Publication Date


  • 2010

Citation


  • Mitchell, T. W., Turner, N., Else, P., Hulbert, A. J., Hawley, J., Lee, J., Bruce, C. & Blanksby, S. J. (2010). The effect of exercise on the skeletal muscle phospholipidome of rats fed a high-fat diet. International Journal of Molecular Sciences, 11 (10), 3954-3964.

Scopus Eid


  • 2-s2.0-79960785642

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1552&context=hbspapers

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/531

Number Of Pages


  • 10

Start Page


  • 3954

End Page


  • 3964

Volume


  • 11

Issue


  • 10

Place Of Publication


  • http://www.mdpi.com/1422-0067/11/10/3954/pdf

Abstract


  • The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague- Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in

    phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.

Authors


  •   Mitchell, Todd
  •   Turner, Nigel (external author)
  •   Else, Paul
  •   Hulbert, Tony J.
  •   Hawley, John A. (external author)
  •   Lee, Jong Sam (external author)
  •   Bruce, Clinton R. (external author)
  •   Blanksby, Stephen J. (external author)

Publication Date


  • 2010

Citation


  • Mitchell, T. W., Turner, N., Else, P., Hulbert, A. J., Hawley, J., Lee, J., Bruce, C. & Blanksby, S. J. (2010). The effect of exercise on the skeletal muscle phospholipidome of rats fed a high-fat diet. International Journal of Molecular Sciences, 11 (10), 3954-3964.

Scopus Eid


  • 2-s2.0-79960785642

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1552&context=hbspapers

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/531

Number Of Pages


  • 10

Start Page


  • 3954

End Page


  • 3964

Volume


  • 11

Issue


  • 10

Place Of Publication


  • http://www.mdpi.com/1422-0067/11/10/3954/pdf