P>Mycoplasma hyopneumoniae, the causative agent of porcine enzootic pneumonia, adheres to ciliated respiratory epithelia resulting in ciliostasis and epithelial cell death. The cilium adhesin P97 (Mhp183) contains two repeat regions, designated R1 and R2, that play key roles in adherence. Eight pentapeptide repeats in R1 are sufficient to bind porcine cilia; however, both R1 and R2 are needed to bind heparin. Mhp271, a paralogue of P97, is the only other M. hyopneumoniae protein to contain both R1 and R2 repeats. These repeats are arranged as a set of three pentapeptide repeats (designated R1A(271)), two decapeptide repeats (designated R2(271)), and a second set of six pentapeptide repeats (designated R1B(271)). To determine their function, recombinant proteins containing R1A(271) (F1(271)) and R2(271)-R1B(271) (F2(271)) were constructed and used in in vitro binding assays. F2(271), but not F1(271), bound heparin (K-D = 8.1 +/- 0.4 nM), fibronectin (K-D = 174 +/- 13 nM) and porcine cilia. Pre-incubation of F2(271) with 100 mu M heparin blocked cilium binding by similar to 69%. Cell surface shaving with trypsin combined with two-dimensional liquid chromatography coupled to tandem mass spectrometry analysis identified Mhp271 as surface-exposed. Our data suggest that both R1 and R2 in Mhp271 are involved in binding to host molecules.