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Molecular cloning and characterization of the global regulator LaeA in Penicillium citrinum

Journal Article


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Abstract


  • We have cloned and analysed a laeA gene (Pci-laeA) that may control mevastatin biosynthesis in Penicillium citrinum. The full-length Pci-laeA sequence is 1,340 bp with an ORF of 1,284 bp encoding 427 amino acids. It shows 95% identity with LaeA from P. chrysogenum. The predicted molecular mass of Pci-LaeA is 48.72 kDa with an estimated theoretical isoelectric point of 6.96. Pci-LaeA has a conserved S-adenosylmethionine binding site and a potential MlcR (a pathway specific regulator in mevastatin biosynthesis) binding site.

Authors


  •   Xing, Wei (external author)
  •   Deng, Chao
  •   Hu, Chang-Hua (external author)

Publication Date


  • 2010

Citation


  • Xing, W., Deng, C. & Hu, C. (2010). Molecular cloning and characterization of the global regulator LaeA in Penicillium citrinum. Biotechnology Letters, 32 (11), 1733-1737.

Scopus Eid


  • 2-s2.0-77957947578

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=4512&context=hbspapers

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/3460

Number Of Pages


  • 4

Start Page


  • 1733

End Page


  • 1737

Volume


  • 32

Issue


  • 11

Place Of Publication


  • http://springerlink.metapress.com/content/g4x28582v43k5658/fulltext.pdf

Abstract


  • We have cloned and analysed a laeA gene (Pci-laeA) that may control mevastatin biosynthesis in Penicillium citrinum. The full-length Pci-laeA sequence is 1,340 bp with an ORF of 1,284 bp encoding 427 amino acids. It shows 95% identity with LaeA from P. chrysogenum. The predicted molecular mass of Pci-LaeA is 48.72 kDa with an estimated theoretical isoelectric point of 6.96. Pci-LaeA has a conserved S-adenosylmethionine binding site and a potential MlcR (a pathway specific regulator in mevastatin biosynthesis) binding site.

Authors


  •   Xing, Wei (external author)
  •   Deng, Chao
  •   Hu, Chang-Hua (external author)

Publication Date


  • 2010

Citation


  • Xing, W., Deng, C. & Hu, C. (2010). Molecular cloning and characterization of the global regulator LaeA in Penicillium citrinum. Biotechnology Letters, 32 (11), 1733-1737.

Scopus Eid


  • 2-s2.0-77957947578

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=4512&context=hbspapers

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/3460

Number Of Pages


  • 4

Start Page


  • 1733

End Page


  • 1737

Volume


  • 32

Issue


  • 11

Place Of Publication


  • http://springerlink.metapress.com/content/g4x28582v43k5658/fulltext.pdf