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Antiplasmodial activity of atisinium chloride from the Bhutanese medicinal plant, Aconitum orochryseum

Journal Article


Abstract


  • Ethnopharmacological relevance: The plant Aconitum orochryseum Stapf. (Ranunculaceae) is employed together with other plants in Bhutanese traditional medicine and is indicated for malaria-associated fever.

    Aim of the study: To study the in vitro antiplasmodial activity of atisinium chloride, the major alkaloid from Aconitum orochryseum.

    Materials and methods: Atisinium chloride was extracted and purified from aerial parts of Aconitum orochryseum and its structure and absolute configuration confirmed by single crystal X-ray crystallography. The crude methanol extract, crude alkaloid fraction, and atisinium chloride were tested for in vitro antiplasmodial activity against the malarial Plasmodium falciparum strains TM4/8.2 (TM4; wild type) and K1CB1 (K1; chloroquine and antifolate resistant).

    Results: The diterpenoid alkaloid atisinium chloride was shown to have moderate antiplasmodial activities with IC50 values of 4 mu M and 3.6 mu M, respectively against the TM4 strain and the K1 strain of Plasmodium falciparum.

    Conclusions: Our studies provide the first evidence in support of one of the indicated treatments with Aconitum orochryseum in Bhutanese traditional medicine. This alkaloid also represents a potential new antimalarial structural lead. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

UOW Authors


  •   Wangchuk, Phurpa (external author)
  •   Bremner, John
  •   Samten, - (external author)
  •   Skelton, Brian W. (external author)
  •   White, Allan H. (external author)
  •   Rattanajak, Roonglawan (external author)
  •   Kamchonwongpaisan, Sumalee (external author)

Publication Date


  • 2010

Citation


  • Wangchuk, P., Bremner, J. B., Samten, , Skelton, B. W., White, A., Rattanajak, R. & Kamchonwongpaisan, S. (2010). Antiplasmodial activity of atisinium chloride from the Bhutanese medicinal plant, Aconitum orochryseum. Journal of Ethnopharmacology, 130 (3), 559-562.

Scopus Eid


  • 2-s2.0-77954956698

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5127

Number Of Pages


  • 3

Start Page


  • 559

End Page


  • 562

Volume


  • 130

Issue


  • 3

Abstract


  • Ethnopharmacological relevance: The plant Aconitum orochryseum Stapf. (Ranunculaceae) is employed together with other plants in Bhutanese traditional medicine and is indicated for malaria-associated fever.

    Aim of the study: To study the in vitro antiplasmodial activity of atisinium chloride, the major alkaloid from Aconitum orochryseum.

    Materials and methods: Atisinium chloride was extracted and purified from aerial parts of Aconitum orochryseum and its structure and absolute configuration confirmed by single crystal X-ray crystallography. The crude methanol extract, crude alkaloid fraction, and atisinium chloride were tested for in vitro antiplasmodial activity against the malarial Plasmodium falciparum strains TM4/8.2 (TM4; wild type) and K1CB1 (K1; chloroquine and antifolate resistant).

    Results: The diterpenoid alkaloid atisinium chloride was shown to have moderate antiplasmodial activities with IC50 values of 4 mu M and 3.6 mu M, respectively against the TM4 strain and the K1 strain of Plasmodium falciparum.

    Conclusions: Our studies provide the first evidence in support of one of the indicated treatments with Aconitum orochryseum in Bhutanese traditional medicine. This alkaloid also represents a potential new antimalarial structural lead. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

UOW Authors


  •   Wangchuk, Phurpa (external author)
  •   Bremner, John
  •   Samten, - (external author)
  •   Skelton, Brian W. (external author)
  •   White, Allan H. (external author)
  •   Rattanajak, Roonglawan (external author)
  •   Kamchonwongpaisan, Sumalee (external author)

Publication Date


  • 2010

Citation


  • Wangchuk, P., Bremner, J. B., Samten, , Skelton, B. W., White, A., Rattanajak, R. & Kamchonwongpaisan, S. (2010). Antiplasmodial activity of atisinium chloride from the Bhutanese medicinal plant, Aconitum orochryseum. Journal of Ethnopharmacology, 130 (3), 559-562.

Scopus Eid


  • 2-s2.0-77954956698

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5127

Number Of Pages


  • 3

Start Page


  • 559

End Page


  • 562

Volume


  • 130

Issue


  • 3