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Anti-inflammatory effects of apolipoprotein A-I in the rabbit

Journal Article


Abstract


  • Objective: Infusions ofapoA-Iinthelipid-freeformorasaconstituentofdiscoidalreconstitutedhigh-density lipoproteins,(A-I)rHDL,markedlyinhibitacutevascularinflammationinnormocholesterolemic New ZealandWhite(NZW)rabbits.ThiseffectisapparentevenwhenapoA-Iisadministered24hpriorto the inflammatoryinsult.Thepresentstudyasksifthisbenefitisrelatedtoanimprovedanti-inflammatory capacity of thehigh-densitylipoprotein(HDL)fraction,ortoincreasedarterialexpressionofgenesthat inhibit inflammation.

    Methods andresults: The abilityofapoA-Itoincreasetheanti-inflammatorycapacityofHDLwasassessed by infusingnormocholesterolemicNZWrabbitswithsaline,lipid-freeapoA-Ior(A-I)rHDL.Theinfused apoA-I incorporatedrapidlyintotherabbitHDLfraction.Theanimalsweresacrificedat5or360minpost-infusion andplasmawascollected.HDLwereisolatedbyultracentrifugationandincubatedwithcytokine-activated culturedhumancoronaryarteryendothelialcells.HDLfromanimalssacrificedat5minpost-apoA-I infusionhadaslightlyenhancedanti-inflammatorycapacityrelativetoHDLfromthesaline- infused animals.Theanti-inflammatorycapacityofHDLfromtheanimalssacrificedat360minpost- apoA-I infusionwascomparabletothatofHDLfromthesaline-infusedanimals.Theeffectof(A-I)rHDL infusions onarterial3-hydroxysteroid-24 reductase(DHCR24)andendothelialadhesionmolecule expression wasinvestigatedincholesterol-fedNZWrabbits.Relativetoanimalsinfusedwithsaline,(A- I)rHDL infusionsdecreasedaorticVCAM-1andICAM-1proteinexpressionby73and54%,respectively (p < 0.05),andincreasedDHCR24mRNAlevelsby56%(p < 0.0001).

UOW Authors


  •   Patel, Sanjay (external author)
  •   Di Bartolo, Belinda A. (external author)
  •   Nakhla, Shirley (external author)
  •   Heather, Alison K. (external author)
  •   Mitchell, Todd
  •   W Jessup, W (external author)
  •   Celermajer, David S. (external author)
  •   Barter, Philip J. (external author)
  •   Rye, Kerry-Anne (external author)

Publication Date


  • 2010

Citation


  • Patel, S., Di Bartolo, B. A., Nakhla, S., Heather, A. K., Mitchell, T. W., Jessup, W., Celermajer, D. S., Barter, P. J. & Rye, K. (2010). Anti-inflammatory effects of apolipoprotein A-I in the rabbit. Atherosclerosis, 212 (2), 392-397.

Scopus Eid


  • 2-s2.0-77957703075

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/3446

Number Of Pages


  • 5

Start Page


  • 392

End Page


  • 397

Volume


  • 212

Issue


  • 2

Place Of Publication


  • http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T12-507CRY0-1-B&_cdi=4878&_user=202616&_pii=S0021915010004132&_origin=browse&_coverDate=10%2F31%2F2010&_sk=997879997&view=c&wchp=dGLbVzW-zSkzV&md5=7bb63a21cc6ed0af62c4f675a3763218&ie=/sdarticle.pdf

Abstract


  • Objective: Infusions ofapoA-Iinthelipid-freeformorasaconstituentofdiscoidalreconstitutedhigh-density lipoproteins,(A-I)rHDL,markedlyinhibitacutevascularinflammationinnormocholesterolemic New ZealandWhite(NZW)rabbits.ThiseffectisapparentevenwhenapoA-Iisadministered24hpriorto the inflammatoryinsult.Thepresentstudyasksifthisbenefitisrelatedtoanimprovedanti-inflammatory capacity of thehigh-densitylipoprotein(HDL)fraction,ortoincreasedarterialexpressionofgenesthat inhibit inflammation.

    Methods andresults: The abilityofapoA-Itoincreasetheanti-inflammatorycapacityofHDLwasassessed by infusingnormocholesterolemicNZWrabbitswithsaline,lipid-freeapoA-Ior(A-I)rHDL.Theinfused apoA-I incorporatedrapidlyintotherabbitHDLfraction.Theanimalsweresacrificedat5or360minpost-infusion andplasmawascollected.HDLwereisolatedbyultracentrifugationandincubatedwithcytokine-activated culturedhumancoronaryarteryendothelialcells.HDLfromanimalssacrificedat5minpost-apoA-I infusionhadaslightlyenhancedanti-inflammatorycapacityrelativetoHDLfromthesaline- infused animals.Theanti-inflammatorycapacityofHDLfromtheanimalssacrificedat360minpost- apoA-I infusionwascomparabletothatofHDLfromthesaline-infusedanimals.Theeffectof(A-I)rHDL infusions onarterial3-hydroxysteroid-24 reductase(DHCR24)andendothelialadhesionmolecule expression wasinvestigatedincholesterol-fedNZWrabbits.Relativetoanimalsinfusedwithsaline,(A- I)rHDL infusionsdecreasedaorticVCAM-1andICAM-1proteinexpressionby73and54%,respectively (p < 0.05),andincreasedDHCR24mRNAlevelsby56%(p < 0.0001).

UOW Authors


  •   Patel, Sanjay (external author)
  •   Di Bartolo, Belinda A. (external author)
  •   Nakhla, Shirley (external author)
  •   Heather, Alison K. (external author)
  •   Mitchell, Todd
  •   W Jessup, W (external author)
  •   Celermajer, David S. (external author)
  •   Barter, Philip J. (external author)
  •   Rye, Kerry-Anne (external author)

Publication Date


  • 2010

Citation


  • Patel, S., Di Bartolo, B. A., Nakhla, S., Heather, A. K., Mitchell, T. W., Jessup, W., Celermajer, D. S., Barter, P. J. & Rye, K. (2010). Anti-inflammatory effects of apolipoprotein A-I in the rabbit. Atherosclerosis, 212 (2), 392-397.

Scopus Eid


  • 2-s2.0-77957703075

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/3446

Number Of Pages


  • 5

Start Page


  • 392

End Page


  • 397

Volume


  • 212

Issue


  • 2

Place Of Publication


  • http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T12-507CRY0-1-B&_cdi=4878&_user=202616&_pii=S0021915010004132&_origin=browse&_coverDate=10%2F31%2F2010&_sk=997879997&view=c&wchp=dGLbVzW-zSkzV&md5=7bb63a21cc6ed0af62c4f675a3763218&ie=/sdarticle.pdf