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Sphingolipid distribution changes with age in the human lens

Journal Article


Abstract


  • The formation of an internal barrier to the diffusion of small molecules in the lens during middle age is hypothesized to be a key event in the development of age-related nuclear (ARN) cataract. Changes in membrane lipids with age may be responsible. In this study, we investigated the effect of age on the distribution of sphingomyelins, the most abundant lens phospholipids. Human lens sections were initially analyzed by MALDI mass spectrometry imaging. A distinct annular distribution of the dihydrosphingomyelin, DHSM (d18:0/16:0), in the barrier region was observed in 64- and 70-year-old lenses but not in a 23- year-old lens. An increase in the dihydroceramide, DHCer (d18:0/16:0), in the lens nucleus was also observed in the older lenses. These fi ndings were supported by ESI mass

    spectrometry analysis of lipid extracts from lenses dissected into outer, barrier, and nuclear regions. A subsequent analysis of 18 lenses ages 20–72 years revealed that sphingomyelin levels increased with age in the barrier region until reaching a plateau at approximately 40 years of age. Such changes in lipid composition will have a signifi cant impact on the physical properties of the fi ber cell membranes and may be associated with the formation of a barrier

UOW Authors


  •   Deeley, Jane M. (external author)
  •   Hankin, Joseph A. (external author)
  •   Friedrich, Michael G. (external author)
  •   Murphy, Robert C. (external author)
  •   Truscott, Roger
  •   Mitchell, Todd
  •   Blanksby, Stephen J. (external author)

Publication Date


  • 2010

Citation


  • Deeley, J., Hankin, J. A., Friedrich, M., Murphy, R. C., Truscott, R. J.W., Mitchell, T. W. & Blanksby, S. J. (2010). Sphingolipid distribution changes with age in the human lens. Journal of Lipid Research, 51 (9), 2753-2760.

Scopus Eid


  • 2-s2.0-77956842668

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5092

Number Of Pages


  • 7

Start Page


  • 2753

End Page


  • 2760

Volume


  • 51

Issue


  • 9

Place Of Publication


  • http://www.jlr.org/content/51/9/2753.full.pdf+html

Abstract


  • The formation of an internal barrier to the diffusion of small molecules in the lens during middle age is hypothesized to be a key event in the development of age-related nuclear (ARN) cataract. Changes in membrane lipids with age may be responsible. In this study, we investigated the effect of age on the distribution of sphingomyelins, the most abundant lens phospholipids. Human lens sections were initially analyzed by MALDI mass spectrometry imaging. A distinct annular distribution of the dihydrosphingomyelin, DHSM (d18:0/16:0), in the barrier region was observed in 64- and 70-year-old lenses but not in a 23- year-old lens. An increase in the dihydroceramide, DHCer (d18:0/16:0), in the lens nucleus was also observed in the older lenses. These fi ndings were supported by ESI mass

    spectrometry analysis of lipid extracts from lenses dissected into outer, barrier, and nuclear regions. A subsequent analysis of 18 lenses ages 20–72 years revealed that sphingomyelin levels increased with age in the barrier region until reaching a plateau at approximately 40 years of age. Such changes in lipid composition will have a signifi cant impact on the physical properties of the fi ber cell membranes and may be associated with the formation of a barrier

UOW Authors


  •   Deeley, Jane M. (external author)
  •   Hankin, Joseph A. (external author)
  •   Friedrich, Michael G. (external author)
  •   Murphy, Robert C. (external author)
  •   Truscott, Roger
  •   Mitchell, Todd
  •   Blanksby, Stephen J. (external author)

Publication Date


  • 2010

Citation


  • Deeley, J., Hankin, J. A., Friedrich, M., Murphy, R. C., Truscott, R. J.W., Mitchell, T. W. & Blanksby, S. J. (2010). Sphingolipid distribution changes with age in the human lens. Journal of Lipid Research, 51 (9), 2753-2760.

Scopus Eid


  • 2-s2.0-77956842668

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5092

Number Of Pages


  • 7

Start Page


  • 2753

End Page


  • 2760

Volume


  • 51

Issue


  • 9

Place Of Publication


  • http://www.jlr.org/content/51/9/2753.full.pdf+html