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Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite

Journal Article


Abstract


  • Electroactivity of polypyrrole hyaluronic acid, electropolymerized in the presence of oxidized carbon nanotubes (PPyHA-CNT) was studied in situ by electrochemical atomic force microscopy (EC-AFM) in physiological electrolyte solution. In situ Raman spectroscopic and quartz crystal microbalance (QCM) studies were conducted on layers of the polymer grown on AT-cut 5 MHz quartz crystals. Human adipose stem cell (ASC) attachment and viability were studied by Live/Dead staining, and the proliferation was evaluated by WST-1 Cell proliferation assay for polypyrrole samples electropolymerized on titanium. According to cyclic voltammetry, the measured specific capacitance of the material on gold is roughly 20% of the reference polypyrrole dodecylbenzene sulfonate (PPyDBS). Electrochemical-QCM (EC-QCM) analysis of a 210-nm thick film reveals that the material is very soft G'similar to 100 kPa and swells upon reduction. EC-AFM of samples polymerized on microelectrodes show that there are areas of varying electroactivity, especially for samples without a hydrophopic backing PPyDBS layer. AFM line scans show typically 20-25% thickness change during electrochemical reduction. Raman spectroscopic analysis suggests that the material supports noticeable polaron conduction. Biocompatibility study of the PPyHA-CNT on titanium with adipose stem cells showed equal or better cell attachment, viability, and proliferation compared with the reference polylactide. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 1056-1067, 2010

UOW Authors


  •   Pelto, Jani M. (external author)
  •   Haimi, Suvi P. (external author)
  •   Puukilainen, Esa (external author)
  •   Whitten, Philip G. (external author)
  •   Spinks, Geoff M.
  •   Bahrami Samani, Mehrdad (external author)
  •   Ritala, Mikko (external author)
  •   Vuorinen, Tommi (external author)

Publication Date


  • 2010

Citation


  • Pelto, J. M., Haimi, S. P., Puukilainen, E., Whitten, P. G., Spinks, G. Maxwell., Bahrami Samani, M., Ritala, M. & Vuorinen, T. (2010). Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite. Journal of Biomedical Materials Research Part A, 93A (3), 1056-1067.

Scopus Eid


  • 2-s2.0-77950640067

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/420

Number Of Pages


  • 11

Start Page


  • 1056

End Page


  • 1067

Volume


  • 93A

Issue


  • 3

Abstract


  • Electroactivity of polypyrrole hyaluronic acid, electropolymerized in the presence of oxidized carbon nanotubes (PPyHA-CNT) was studied in situ by electrochemical atomic force microscopy (EC-AFM) in physiological electrolyte solution. In situ Raman spectroscopic and quartz crystal microbalance (QCM) studies were conducted on layers of the polymer grown on AT-cut 5 MHz quartz crystals. Human adipose stem cell (ASC) attachment and viability were studied by Live/Dead staining, and the proliferation was evaluated by WST-1 Cell proliferation assay for polypyrrole samples electropolymerized on titanium. According to cyclic voltammetry, the measured specific capacitance of the material on gold is roughly 20% of the reference polypyrrole dodecylbenzene sulfonate (PPyDBS). Electrochemical-QCM (EC-QCM) analysis of a 210-nm thick film reveals that the material is very soft G'similar to 100 kPa and swells upon reduction. EC-AFM of samples polymerized on microelectrodes show that there are areas of varying electroactivity, especially for samples without a hydrophopic backing PPyDBS layer. AFM line scans show typically 20-25% thickness change during electrochemical reduction. Raman spectroscopic analysis suggests that the material supports noticeable polaron conduction. Biocompatibility study of the PPyHA-CNT on titanium with adipose stem cells showed equal or better cell attachment, viability, and proliferation compared with the reference polylactide. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 1056-1067, 2010

UOW Authors


  •   Pelto, Jani M. (external author)
  •   Haimi, Suvi P. (external author)
  •   Puukilainen, Esa (external author)
  •   Whitten, Philip G. (external author)
  •   Spinks, Geoff M.
  •   Bahrami Samani, Mehrdad (external author)
  •   Ritala, Mikko (external author)
  •   Vuorinen, Tommi (external author)

Publication Date


  • 2010

Citation


  • Pelto, J. M., Haimi, S. P., Puukilainen, E., Whitten, P. G., Spinks, G. Maxwell., Bahrami Samani, M., Ritala, M. & Vuorinen, T. (2010). Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite. Journal of Biomedical Materials Research Part A, 93A (3), 1056-1067.

Scopus Eid


  • 2-s2.0-77950640067

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/420

Number Of Pages


  • 11

Start Page


  • 1056

End Page


  • 1067

Volume


  • 93A

Issue


  • 3