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Hydration dependent studies of highly aligned multilayer lipid membranes by neutron scattering

Journal Article


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Abstract


  • We investigated molecular motions on a picoseconds timescale of 1,2-Dimyristoyl-sn-

    Glycero-3-Phosphocholine (DMPC) model membranes as a function of hydration by using

    elastic and quasielastic neutron scattering. Two different hydrations corresponding to

    approximately nine and twelve water molecules per lipid were studied, the latter being

    the fully hydrated state. In our study we focused on head group motions by using chain

    deuterated lipids. Information on in-plane and out-of-plane motions could be extracted

    by using solid supported DMPC multilayers. Our studies confirm and complete former

    investigations by K¨onig et al. and Rheinst¨adter et al. who describe the dynamics of lipidmembranes,

    but did not explore the influence of hydration on the head group dynamics as

    presented here. From the elastic data a clear shift of the main phase transition from the

    Pβ ripple phase to the Lα liquid phase was observed. A decreasing water content moves

    the transition temperature to higher temperatures. The quasielastic data permit a closer

    investigation of the different types of head group motion of the two samples. Two different

    model are needed to fit the elastic incoherent structure factor (EISF) and corresponding

    radii were calculated.

    Therefore the presented data show the strong influence hydration has on the head group

    mobility of DMPC.

UOW Authors


  •   Trapp, Marcus (external author)
  •   Gutberlet, Thomas (external author)
  •   Juranyi, Fanni (external author)
  •   Unruh, T (external author)
  •   Demé, Bruno (external author)
  •   Tehei, Moeava
  •   Peters, Judith (external author)

Publication Date


  • 2010

Citation


  • Trapp, M., Gutberlet, T., Juranyi, F., Unruh, T., Demé, B., Tehei, M. & Peters, J. (2010). Hydration dependent studies of highly aligned multilayer lipid membranes by neutron scattering. Journal of Chemical Physics, 133 (16), 164505-1-164505-7.

Scopus Eid


  • 2-s2.0-78149367351

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1556&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/517

Start Page


  • 164505-1

End Page


  • 164505-7

Volume


  • 133

Issue


  • 16

Place Of Publication


  • http://jcp.aip.org/

Abstract


  • We investigated molecular motions on a picoseconds timescale of 1,2-Dimyristoyl-sn-

    Glycero-3-Phosphocholine (DMPC) model membranes as a function of hydration by using

    elastic and quasielastic neutron scattering. Two different hydrations corresponding to

    approximately nine and twelve water molecules per lipid were studied, the latter being

    the fully hydrated state. In our study we focused on head group motions by using chain

    deuterated lipids. Information on in-plane and out-of-plane motions could be extracted

    by using solid supported DMPC multilayers. Our studies confirm and complete former

    investigations by K¨onig et al. and Rheinst¨adter et al. who describe the dynamics of lipidmembranes,

    but did not explore the influence of hydration on the head group dynamics as

    presented here. From the elastic data a clear shift of the main phase transition from the

    Pβ ripple phase to the Lα liquid phase was observed. A decreasing water content moves

    the transition temperature to higher temperatures. The quasielastic data permit a closer

    investigation of the different types of head group motion of the two samples. Two different

    model are needed to fit the elastic incoherent structure factor (EISF) and corresponding

    radii were calculated.

    Therefore the presented data show the strong influence hydration has on the head group

    mobility of DMPC.

UOW Authors


  •   Trapp, Marcus (external author)
  •   Gutberlet, Thomas (external author)
  •   Juranyi, Fanni (external author)
  •   Unruh, T (external author)
  •   Demé, Bruno (external author)
  •   Tehei, Moeava
  •   Peters, Judith (external author)

Publication Date


  • 2010

Citation


  • Trapp, M., Gutberlet, T., Juranyi, F., Unruh, T., Demé, B., Tehei, M. & Peters, J. (2010). Hydration dependent studies of highly aligned multilayer lipid membranes by neutron scattering. Journal of Chemical Physics, 133 (16), 164505-1-164505-7.

Scopus Eid


  • 2-s2.0-78149367351

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1556&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/517

Start Page


  • 164505-1

End Page


  • 164505-7

Volume


  • 133

Issue


  • 16

Place Of Publication


  • http://jcp.aip.org/