Hydration dependent studies of highly aligned multilayer lipid membranes by neutron scattering

We investigated molecular motions on a picoseconds timescale of 1,2-Dimyristoyl-sn-

Glycero-3-Phosphocholine (DMPC) model membranes as a function of hydration by using

elastic and quasielastic neutron scattering. Two different hydrations corresponding to

approximately nine and twelve water molecules per lipid were studied, the latter being

the fully hydrated state. In our study we focused on head group motions by using chain

deuterated lipids. Information on in-plane and out-of-plane motions could be extracted

by using solid supported DMPC multilayers. Our studies confirm and complete former

investigations by K¨onig et al. and Rheinst¨adter et al. who describe the dynamics of lipidmembranes,

but did not explore the influence of hydration on the head group dynamics as

presented here. From the elastic data a clear shift of the main phase transition from the

Pβ ripple phase to the Lα liquid phase was observed. A decreasing water content moves

the transition temperature to higher temperatures. The quasielastic data permit a closer

investigation of the different types of head group motion of the two samples. Two different

model are needed to fit the elastic incoherent structure factor (EISF) and corresponding

radii were calculated.

Therefore the presented data show the strong influence hydration has on the head group

mobility of DMPC.