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Specific cellular water dynamics observed in vivo by neutron scattering and NMR.

Journal Article


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Abstract


  • Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics,

    measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula

    marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely

    permits the determination of motions on the molecular length (Ba˚ ngstrøm) and time

    (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the

    hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid

    water. It is not ‘‘tamed’’ by confinement. In contrast, in the extreme halophile archaeon,

    in addition to free and hydration water an intracellular water component was observed with

    significantly slowed down translational diffusion. The results are discussed and compared to

    observations in E. coli and Haloarcula marismortui by deuteron spin relaxation in NMR—a

    method that is sensitive to water rotational dynamics on a wide range of time scales.

Authors


  •   Tehei, Moeava
  •   Jasnin, Marion (external author)
  •   Stadler, Andreas (external author)
  •   Zaccai, Giuseppe (external author)

Publication Date


  • 2010

Citation


  • Tehei, M., Jasnin, M., Stadler, A. & Zaccai, G. (2010). Specific cellular water dynamics observed in vivo by neutron scattering and NMR.. Physical Chemistry Chemical Physics, 12 (35), 10154-10160.

Scopus Eid


  • 2-s2.0-77958021289

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1540&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/501

Number Of Pages


  • 6

Start Page


  • 10154

End Page


  • 10160

Volume


  • 12

Issue


  • 35

Abstract


  • Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics,

    measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula

    marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely

    permits the determination of motions on the molecular length (Ba˚ ngstrøm) and time

    (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the

    hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid

    water. It is not ‘‘tamed’’ by confinement. In contrast, in the extreme halophile archaeon,

    in addition to free and hydration water an intracellular water component was observed with

    significantly slowed down translational diffusion. The results are discussed and compared to

    observations in E. coli and Haloarcula marismortui by deuteron spin relaxation in NMR—a

    method that is sensitive to water rotational dynamics on a wide range of time scales.

Authors


  •   Tehei, Moeava
  •   Jasnin, Marion (external author)
  •   Stadler, Andreas (external author)
  •   Zaccai, Giuseppe (external author)

Publication Date


  • 2010

Citation


  • Tehei, M., Jasnin, M., Stadler, A. & Zaccai, G. (2010). Specific cellular water dynamics observed in vivo by neutron scattering and NMR.. Physical Chemistry Chemical Physics, 12 (35), 10154-10160.

Scopus Eid


  • 2-s2.0-77958021289

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1540&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/501

Number Of Pages


  • 6

Start Page


  • 10154

End Page


  • 10160

Volume


  • 12

Issue


  • 35