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Dietary fish oil reduces skeletal muscle oxygen consumption, provides fatigue resistance and improves contractile recovery in the rat in vivo hindlimb

Journal Article


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Abstract


  • Dietary fish oil modulates skeletal muscle membrane fatty acid composition. Similar changes in heart membrane composition modulate myocardial oxygen consumption and enhance mechanical performance. The rat in vivo autologous perfused hindlimb was used to investigate the influence of membrane composition on skeletal muscle function. Male Wistar rats were fed either saturated fat (SF), n-6 PUFA (linoleic acid rich) or n-3 PUFA (fish oil) diets for 8 weeks. Hindlimb skeletal muscle perfused using the animal’s own blood was stimulated via the sciatic nerve (1 Hz, 6-12 V, 0·05 ms) to contract in repeated 10 min bouts. The n-3 PUFA diet markedly increased 22 : 6n-3 DHA, total n-3 PUFA and

    decreased the n-6:n-3 PUFA ratio (P,0·05) in red and white skeletal muscle membranes. There was no difference in initial twitch tension but the n-3 PUFA group maintained greater twitch tension within all contraction bouts and recovered better during rest to produce greater twitch tension throughout the final contraction bout (P,0·05). Hindlimb oxygen consumption during contraction was significantly lower in the n-3 PUFA group compared with the SF group, producing a significantly higher O2 efficiency index compared with both SF and n-6 PUFA groups (P,0·05). Resting oxygen consumption was increased in recovery in the SF group (P,0·05) but did not change in the n-3 PUFA group. Membrane incorporation of n-3 PUFA DHA following fish oil feeding was associated with increased efficiency of muscle O2 consumption and promoted resistance to muscle fatigue.

Publication Date


  • 2010

Citation


  • Peoples, G. E. & McLennan, P. L. (2010). Dietary fish oil reduces skeletal muscle oxygen consumption, provides fatigue resistance and improves contractile recovery in the rat in vivo hindlimb. The British Journal of Nutrition: an international journal of nutritional science, 104 (12), 1771-1779.

Scopus Eid


  • 2-s2.0-78650003869

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1547&context=hbspapers

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/526

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 1771

End Page


  • 1779

Volume


  • 104

Issue


  • 12

Abstract


  • Dietary fish oil modulates skeletal muscle membrane fatty acid composition. Similar changes in heart membrane composition modulate myocardial oxygen consumption and enhance mechanical performance. The rat in vivo autologous perfused hindlimb was used to investigate the influence of membrane composition on skeletal muscle function. Male Wistar rats were fed either saturated fat (SF), n-6 PUFA (linoleic acid rich) or n-3 PUFA (fish oil) diets for 8 weeks. Hindlimb skeletal muscle perfused using the animal’s own blood was stimulated via the sciatic nerve (1 Hz, 6-12 V, 0·05 ms) to contract in repeated 10 min bouts. The n-3 PUFA diet markedly increased 22 : 6n-3 DHA, total n-3 PUFA and

    decreased the n-6:n-3 PUFA ratio (P,0·05) in red and white skeletal muscle membranes. There was no difference in initial twitch tension but the n-3 PUFA group maintained greater twitch tension within all contraction bouts and recovered better during rest to produce greater twitch tension throughout the final contraction bout (P,0·05). Hindlimb oxygen consumption during contraction was significantly lower in the n-3 PUFA group compared with the SF group, producing a significantly higher O2 efficiency index compared with both SF and n-6 PUFA groups (P,0·05). Resting oxygen consumption was increased in recovery in the SF group (P,0·05) but did not change in the n-3 PUFA group. Membrane incorporation of n-3 PUFA DHA following fish oil feeding was associated with increased efficiency of muscle O2 consumption and promoted resistance to muscle fatigue.

Publication Date


  • 2010

Citation


  • Peoples, G. E. & McLennan, P. L. (2010). Dietary fish oil reduces skeletal muscle oxygen consumption, provides fatigue resistance and improves contractile recovery in the rat in vivo hindlimb. The British Journal of Nutrition: an international journal of nutritional science, 104 (12), 1771-1779.

Scopus Eid


  • 2-s2.0-78650003869

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1547&context=hbspapers

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/526

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 1771

End Page


  • 1779

Volume


  • 104

Issue


  • 12