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Membrane fatty acid composition of rat skeletal muscle is most responsive to the balance of dietary n-3 and n-6 PUFA

Journal Article


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Abstract


  • The present study quantifies the relationships between diet fatty acid profile and fatty acid composition of rat skeletal muscle phospholipids. Young adult male Sprague–Dawley rats were fed, for 8 weeks, on one of twelve moderate-fat diets (25% of total energy) differing only in fatty acid profile. SFA content ranged from 8–88% of total fatty acids, MUFA 6–65 %, total PUFA 4–81 %, n-6 PUFA 3–70% and n-3 PUFA 1–70 %. Diet PUFA included only essential fatty acids 18 : 2n-6 and 18 : 3n-3. The balance between n-3 and n-6 PUFA (PUFA balance) in the diet ranged from 1 : 99 to 86 : 14% n-3 PUFA:n-6 PUFA. The slope of muscle phospholipid composition plotted against diet composition quantifies the response of muscle membrane composition to dietary fat (0, no response; 1, complete conformity with diet). The resulting slopes were 0·02 (SFA), 0·10 (PUFA), 0·11 (MUFA), 0·14 (n-3 PUFA) and 0·23 (n-6 PUFA). The response to PUFA balance was biphasic with a slope of 0·98 below 10% diet PUFA balance and 0·16 above 10 %. Thus, low diet PUFA balance has greater influence on muscle composition than

    18-carbon n-3 or n-6 PUFA individually. Equations provided may allow prediction of muscle composition for other diet studies. Diet PUFA balance dramatically affects muscle 20 : 4n-6 and 22 : 6n-3. This may have significant implications for some disease states in human subjects.

Publication Date


  • 2010

Citation


  • Abbott, S. K., Else, P. & Hulbert, A. J. (2010). Membrane fatty acid composition of rat skeletal muscle is most responsive to the balance of dietary n-3 and n-6 PUFA. The British Journal of Nutrition: an international journal of nutritional science, 103 (4), 522-529.

Scopus Eid


  • 2-s2.0-77649221840

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=4458&context=hbspapers

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/3406

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 522

End Page


  • 529

Volume


  • 103

Issue


  • 4

Place Of Publication


  • United Kingdom

Abstract


  • The present study quantifies the relationships between diet fatty acid profile and fatty acid composition of rat skeletal muscle phospholipids. Young adult male Sprague–Dawley rats were fed, for 8 weeks, on one of twelve moderate-fat diets (25% of total energy) differing only in fatty acid profile. SFA content ranged from 8–88% of total fatty acids, MUFA 6–65 %, total PUFA 4–81 %, n-6 PUFA 3–70% and n-3 PUFA 1–70 %. Diet PUFA included only essential fatty acids 18 : 2n-6 and 18 : 3n-3. The balance between n-3 and n-6 PUFA (PUFA balance) in the diet ranged from 1 : 99 to 86 : 14% n-3 PUFA:n-6 PUFA. The slope of muscle phospholipid composition plotted against diet composition quantifies the response of muscle membrane composition to dietary fat (0, no response; 1, complete conformity with diet). The resulting slopes were 0·02 (SFA), 0·10 (PUFA), 0·11 (MUFA), 0·14 (n-3 PUFA) and 0·23 (n-6 PUFA). The response to PUFA balance was biphasic with a slope of 0·98 below 10% diet PUFA balance and 0·16 above 10 %. Thus, low diet PUFA balance has greater influence on muscle composition than

    18-carbon n-3 or n-6 PUFA individually. Equations provided may allow prediction of muscle composition for other diet studies. Diet PUFA balance dramatically affects muscle 20 : 4n-6 and 22 : 6n-3. This may have significant implications for some disease states in human subjects.

Publication Date


  • 2010

Citation


  • Abbott, S. K., Else, P. & Hulbert, A. J. (2010). Membrane fatty acid composition of rat skeletal muscle is most responsive to the balance of dietary n-3 and n-6 PUFA. The British Journal of Nutrition: an international journal of nutritional science, 103 (4), 522-529.

Scopus Eid


  • 2-s2.0-77649221840

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=4458&context=hbspapers

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/3406

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 522

End Page


  • 529

Volume


  • 103

Issue


  • 4

Place Of Publication


  • United Kingdom