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Online ozonolysis methods for the determination of double bond position in unsaturated lipids

Journal Article


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Abstract


  • Modern lipidomics relies heavily on mass spectrometry for the structural characterization and quantification of lipids of biological origins. Structural information is gained by tandem mass spectrometry (MS/MS) whereby lipid ions are fragmented to elucidate lipid class, fatty acid chain length, and degree of unsaturation. Unfortunately, however, in most cases double bond position cannot be assigned based on MS/MS data alone and thus significant structural diversity is hidden from such analyses. For this reason, we have developed two online methods for determining double bond position within unsaturated lipids; ozone electrospray ionization mass spectrometry (OzESI-MS) and ozone-induced dissociation (OzID). Both techniques utilize ozone to cleave C-C double bonds that result in chemically induced fragment ions that locate the position(s) of unsaturation.

UOW Authors


  •   Thomas, Michael C. (external author)
  •   Mitchell, Todd
  •   Blanksby, Stephen J. (external author)

Publication Date


  • 2009

Citation


  • Thomas, M., Mitchell, T. W. & Blanksby, S. J. (2009). Online ozonolysis methods for the determination of double bond position in unsaturated lipids. Methods in Molecular Biology, 579 413-441.

Scopus Eid


  • 2-s2.0-74949103391

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1316&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/282

Number Of Pages


  • 28

Start Page


  • 413

End Page


  • 441

Volume


  • 579

Abstract


  • Modern lipidomics relies heavily on mass spectrometry for the structural characterization and quantification of lipids of biological origins. Structural information is gained by tandem mass spectrometry (MS/MS) whereby lipid ions are fragmented to elucidate lipid class, fatty acid chain length, and degree of unsaturation. Unfortunately, however, in most cases double bond position cannot be assigned based on MS/MS data alone and thus significant structural diversity is hidden from such analyses. For this reason, we have developed two online methods for determining double bond position within unsaturated lipids; ozone electrospray ionization mass spectrometry (OzESI-MS) and ozone-induced dissociation (OzID). Both techniques utilize ozone to cleave C-C double bonds that result in chemically induced fragment ions that locate the position(s) of unsaturation.

UOW Authors


  •   Thomas, Michael C. (external author)
  •   Mitchell, Todd
  •   Blanksby, Stephen J. (external author)

Publication Date


  • 2009

Citation


  • Thomas, M., Mitchell, T. W. & Blanksby, S. J. (2009). Online ozonolysis methods for the determination of double bond position in unsaturated lipids. Methods in Molecular Biology, 579 413-441.

Scopus Eid


  • 2-s2.0-74949103391

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1316&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/282

Number Of Pages


  • 28

Start Page


  • 413

End Page


  • 441

Volume


  • 579