Skip to main content

Skeletal muscle cell proliferation and differentiation on polypyrrole substrates doped with extracellular matrix components

Journal Article


Abstract


  • Conducting polymers have been developed as substrates for in vitro studies with a range of cell types including electrically-excitable cells such as nerve and smooth muscle. The goal of this study was to optimise and characterise a range of polypyrrole materials to act as substrates for electrical stimulation of differentiating skeletal myoblasts. Although all of the polymer materials provided suitable substrates for myoblast adhesion and proliferation, significant differences became apparent under the low-serum conditions used for differentiation of primary myoblasts. The significance of the work lies in the design and control of polymer materials to facilitate different stages of skeletal muscle cell proliferation and/or differentiation, opening up opportunities for engineering of this tissue. This paper therefore constitutes not just a biocompatibility assessment but a comprehensive study of how synthesis conditions affect the final outcome in terms of cell response.

UOW Authors


  •   Gilmore, Kerry J. (external author)
  •   Kita, Magdalena (external author)
  •   Wallace, Gordon
  •   Kapsa, Robert
  •   Higgins, Michael
  •   Han, Yao (external author)
  •   Gelmi, Amy A. (external author)
  •   Moulton, Simon E. (external author)
  •   Clark, Graeme M. (external author)

Publication Date


  • 2009

Citation


  • Gilmore, K. J., Kita, M., Wallace, G. G., Kapsa, R., Higgins, M. J., Han, Y., Gelmi, A., Moulton, S. E. & Clark, G. (2009). Skeletal muscle cell proliferation and differentiation on polypyrrole substrates doped with extracellular matrix components. Biomaterials, 30 (29), 5292-5304.

Scopus Eid


  • 2-s2.0-68549097793

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/388

Has Global Citation Frequency


Number Of Pages


  • 12

Start Page


  • 5292

End Page


  • 5304

Volume


  • 30

Issue


  • 29

Abstract


  • Conducting polymers have been developed as substrates for in vitro studies with a range of cell types including electrically-excitable cells such as nerve and smooth muscle. The goal of this study was to optimise and characterise a range of polypyrrole materials to act as substrates for electrical stimulation of differentiating skeletal myoblasts. Although all of the polymer materials provided suitable substrates for myoblast adhesion and proliferation, significant differences became apparent under the low-serum conditions used for differentiation of primary myoblasts. The significance of the work lies in the design and control of polymer materials to facilitate different stages of skeletal muscle cell proliferation and/or differentiation, opening up opportunities for engineering of this tissue. This paper therefore constitutes not just a biocompatibility assessment but a comprehensive study of how synthesis conditions affect the final outcome in terms of cell response.

UOW Authors


  •   Gilmore, Kerry J. (external author)
  •   Kita, Magdalena (external author)
  •   Wallace, Gordon
  •   Kapsa, Robert
  •   Higgins, Michael
  •   Han, Yao (external author)
  •   Gelmi, Amy A. (external author)
  •   Moulton, Simon E. (external author)
  •   Clark, Graeme M. (external author)

Publication Date


  • 2009

Citation


  • Gilmore, K. J., Kita, M., Wallace, G. G., Kapsa, R., Higgins, M. J., Han, Y., Gelmi, A., Moulton, S. E. & Clark, G. (2009). Skeletal muscle cell proliferation and differentiation on polypyrrole substrates doped with extracellular matrix components. Biomaterials, 30 (29), 5292-5304.

Scopus Eid


  • 2-s2.0-68549097793

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/388

Has Global Citation Frequency


Number Of Pages


  • 12

Start Page


  • 5292

End Page


  • 5304

Volume


  • 30

Issue


  • 29