Skip to main content
placeholder image

Model for amorphous aggregation processes

Journal Article


Download full-text (Open Access)

Abstract


  • The amorphous aggregation of proteins is associated with many phenomena, ranging from the formation of protein wine haze to the development of cataract in the eye lens and the precipitation of recombinant proteins during their expression and purification. While much literature exists describing models for linear protein aggregation, such as amyloid fibril formation, there are few reports of models which address amorphous aggregation. Here, we propose a model to describe the amorphous aggregation of proteins which is also more widely applicable to other situations where a similar process occurs, such as in the formation of colloids and nanoclusters. As first applications of the model, we have tested it against experimental turbidimetry data of three proteins relevant to the wine industry and biochemistry, namely, thaumatin, a thaumatinlike protein, and alpha-lactalbumin. The model is very robust and describes amorphous experimental data to a high degree of accuracy. Details about the aggregation process, such as shape parameters of the aggregates and rate constants, can also be extracted.

Authors


  •   Stranks, Samual (external author)
  •   Ecroyd, Heath
  •   Van Sluyter, Steve (external author)
  •   Waters, Elizabeth J. (external author)
  •   Carver, John A. (external author)
  •   von Smekal, Lorenz (external author)

Publication Date


  • 2009

Citation


  • Stranks, S., Ecroyd, H., Van Sluyter, S., Waters, E. J., Carver, J. A. & von Smekal, L. (2009). Model for amorphous aggregation processes. Physical Review E: Statistical, Nonlinear, and Soft Matter Physics, 80 (5), 051907-1-051907-13.

Scopus Eid


  • 2-s2.0-71049157206

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1256&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/222

Has Global Citation Frequency


Start Page


  • 051907-1

End Page


  • 051907-13

Volume


  • 80

Issue


  • 5

Abstract


  • The amorphous aggregation of proteins is associated with many phenomena, ranging from the formation of protein wine haze to the development of cataract in the eye lens and the precipitation of recombinant proteins during their expression and purification. While much literature exists describing models for linear protein aggregation, such as amyloid fibril formation, there are few reports of models which address amorphous aggregation. Here, we propose a model to describe the amorphous aggregation of proteins which is also more widely applicable to other situations where a similar process occurs, such as in the formation of colloids and nanoclusters. As first applications of the model, we have tested it against experimental turbidimetry data of three proteins relevant to the wine industry and biochemistry, namely, thaumatin, a thaumatinlike protein, and alpha-lactalbumin. The model is very robust and describes amorphous experimental data to a high degree of accuracy. Details about the aggregation process, such as shape parameters of the aggregates and rate constants, can also be extracted.

Authors


  •   Stranks, Samual (external author)
  •   Ecroyd, Heath
  •   Van Sluyter, Steve (external author)
  •   Waters, Elizabeth J. (external author)
  •   Carver, John A. (external author)
  •   von Smekal, Lorenz (external author)

Publication Date


  • 2009

Citation


  • Stranks, S., Ecroyd, H., Van Sluyter, S., Waters, E. J., Carver, J. A. & von Smekal, L. (2009). Model for amorphous aggregation processes. Physical Review E: Statistical, Nonlinear, and Soft Matter Physics, 80 (5), 051907-1-051907-13.

Scopus Eid


  • 2-s2.0-71049157206

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1256&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/222

Has Global Citation Frequency


Start Page


  • 051907-1

End Page


  • 051907-13

Volume


  • 80

Issue


  • 5