Skip to main content
placeholder image

Correlation between HIV-1 RNA load in blood and seminal plasma depending on antiretroviral treatment status, regimen and penetration of semen by antiretroviral drugs

Journal Article


Abstract


  • To assess the correlation between HIV-1 RNA load in blood and semen by

    antiretroviral therapy status and the relative penetration of antiretroviral

    drugs in seminal plasma. We performed a cross-sectional cohort study of 119 HIV-1

    subjects divided into three groups according to treatment status. Blood and semen

    were collected concurrently. Seminal viral load determined by NucliSens HIV-1 QT

    PCR (BioMerieux). Viral suppression over time was assessed in a second semen

    sample collected from 10 treated subjects. Antiretroviral plasma concentrations

    were measured by high performance liquid chromatography and recovery experiments

    were performed on semen samples to validate quantitation in this matrix. All

    subjects taking non nucleoside reverse transcriptase inhibitors (n = 36, mean

    treatment 33 months +/- 14) or protease inhibitors (n = 45, mean treatment 31

    months +/- 25) had blood viral load < 50 copies/mL and seminal viral load < 250

    copies/mL. In untreated subjects (n = 38), blood and semen viral loads were

    positively correlated (Spearman's rho = 0.489, p = 0.002). Blood and semen

    nevirapine concentrations were positively correlated (r(2) = 0.795, p = 0.005)

    and therapeutic concentrations achieved in both compartments. Lopinavir and

    atazanavir also penetrated semen but efavirenz did not. We find that there is

    compartmentalisation of HIV-1 within the male genital tract and propose that new

    infections may originate from untreated men and that suppressive antiretroviral

    regimens may reduce the risk of sexual transmission.

Authors


  •   Chan, Derek J. (external author)
  •   Ray, J E. (external author)
  •   McNally, Leon (external author)
  •   Batterham, Marijka
  •   Smith, Don E. (external author)

Publication Date


  • 2008

Citation


  • Chan, D., Ray, J. E., McNally, L., Batterham, M. & Smith, D. E. (2008). Correlation between HIV-1 RNA load in blood and seminal plasma depending on antiretroviral treatment status, regimen and penetration of semen by antiretroviral drugs. Current HIV Research, 6 (5), 477-484.

Scopus Eid


  • 2-s2.0-53849110624

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/1700

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 477

End Page


  • 484

Volume


  • 6

Issue


  • 5

Place Of Publication


  • Netherlands

Abstract


  • To assess the correlation between HIV-1 RNA load in blood and semen by

    antiretroviral therapy status and the relative penetration of antiretroviral

    drugs in seminal plasma. We performed a cross-sectional cohort study of 119 HIV-1

    subjects divided into three groups according to treatment status. Blood and semen

    were collected concurrently. Seminal viral load determined by NucliSens HIV-1 QT

    PCR (BioMerieux). Viral suppression over time was assessed in a second semen

    sample collected from 10 treated subjects. Antiretroviral plasma concentrations

    were measured by high performance liquid chromatography and recovery experiments

    were performed on semen samples to validate quantitation in this matrix. All

    subjects taking non nucleoside reverse transcriptase inhibitors (n = 36, mean

    treatment 33 months +/- 14) or protease inhibitors (n = 45, mean treatment 31

    months +/- 25) had blood viral load < 50 copies/mL and seminal viral load < 250

    copies/mL. In untreated subjects (n = 38), blood and semen viral loads were

    positively correlated (Spearman's rho = 0.489, p = 0.002). Blood and semen

    nevirapine concentrations were positively correlated (r(2) = 0.795, p = 0.005)

    and therapeutic concentrations achieved in both compartments. Lopinavir and

    atazanavir also penetrated semen but efavirenz did not. We find that there is

    compartmentalisation of HIV-1 within the male genital tract and propose that new

    infections may originate from untreated men and that suppressive antiretroviral

    regimens may reduce the risk of sexual transmission.

Authors


  •   Chan, Derek J. (external author)
  •   Ray, J E. (external author)
  •   McNally, Leon (external author)
  •   Batterham, Marijka
  •   Smith, Don E. (external author)

Publication Date


  • 2008

Citation


  • Chan, D., Ray, J. E., McNally, L., Batterham, M. & Smith, D. E. (2008). Correlation between HIV-1 RNA load in blood and seminal plasma depending on antiretroviral treatment status, regimen and penetration of semen by antiretroviral drugs. Current HIV Research, 6 (5), 477-484.

Scopus Eid


  • 2-s2.0-53849110624

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/1700

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 477

End Page


  • 484

Volume


  • 6

Issue


  • 5

Place Of Publication


  • Netherlands