Food portion images have been shown to enhance the accuracy of dietary reporting. The current food portion images on the DietAdvice, a website for self-administered recall of usual intake, have been well received by users during pilot testing who reported the visual aid to be more useful than text only portion sizing. The current images are limited and do not contain a standard of reference, which is required as the images are not shown in their actual size on screen. The aim of this study is to further the development of the DietAdvice website by updating the website portion images for commercial acceptability. Foods contained in the DietAdvice website have been assessed and 200 food items requiring food portion images identified. Portion sizes range from 4 to 6 images per food item. The food items vary from single food through to cooked dishes and beverages and needed to be rank ordered prior to photographs being taken. Foods were prepared and photographed by a professional photographer following criteria from the literature for food portion sizing including a standard of reference and uploaded to the website. Automation of dietary processes requires highly standardised visual aids for food portion sizing as human computer interactions differ from face-to-face interaction with each user potentially having a different interpretation of the size.