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Human lens lipids differ markedly from those of commonly used experimental animals

Journal Article


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Abstract


  • Electrospray ionisation tandem mass spectrometry has allowed the unambiguous identification and

    quantification of individual lens phospholipids in human and six animal models. Using this approach ca. 100

    unique phospholipids have been characterised. Parallel analysis of the same lens extracts by a novel directinsertion

    electron-ionization technique found the cholesterol content of human lenses to be significantly

    higher (ca. 6 times) than lenses from the other animals.

    The most abundant phospholipids in all the lenses examined were choline-containing phospholipids. In rat,

    mouse, sheep, cow, pig and chicken, thesewere present largely as phosphatidylcholines, in contrast 66% of the

    total phospholipid in Homo sapienswas sphingomyelin, with the most abundant being dihydrosphingomyelins,

    in particular SM(d18:0/16:0) and SM(d18:0/24:1). The abundant glycerophospholipids within human lenses

    were found to be predominantly phosphatidylethanolamines and phosphatidylserines with surprisingly high

    concentrations of ether-linked alkyl chains identified in both classes. This study is the first to identify the

    phospholipid class (head-group) and assign the constituent fatty acid(s) for each lipid molecule and to quantify

    individual lens phospholipids using internal standards. These data clearly indicate marked differences in the

    membrane lipid composition of the human lens compared to commonly used animal models and thus predict a

    significant variation in the membrane properties of human lens fibre cells compared to those of other animals.

UOW Authors


  •   Deeley, Jane M. (external author)
  •   Mitchell, Todd
  •   Wei, Xiaojia Eric. (external author)
  •   Korth, John (external author)
  •   Nealon, Jessica
  •   Blanksby, Stephen J. (external author)
  •   Truscott, Roger

Publication Date


  • 2008

Citation


  • Deeley, J. M., Mitchell, T. W., Wei, X., Korth, J., Hughes, J. R., Blanksby, S. J. & Truscott, R. J.W. (2008). Human lens lipids differ markedly from those of commonly used experimental animals. BBA - Molecular and Cell Biology of Lipids, 1781 (6-7), 288-298.

Scopus Eid


  • 2-s2.0-48749119985

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3077&context=hbspapers

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/2024

Number Of Pages


  • 10

Start Page


  • 288

End Page


  • 298

Volume


  • 1781

Issue


  • 6-7

Place Of Publication


  • http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%236183%232008%23982189993%23692912%23FLA%23&_cdi=6183&_pubType=J&_auth=y&_acct=C000014118&_version=1&_urlVersion=0&_userid=202616&md5=22a456dd39170baba69ed19f18d2ff89

Abstract


  • Electrospray ionisation tandem mass spectrometry has allowed the unambiguous identification and

    quantification of individual lens phospholipids in human and six animal models. Using this approach ca. 100

    unique phospholipids have been characterised. Parallel analysis of the same lens extracts by a novel directinsertion

    electron-ionization technique found the cholesterol content of human lenses to be significantly

    higher (ca. 6 times) than lenses from the other animals.

    The most abundant phospholipids in all the lenses examined were choline-containing phospholipids. In rat,

    mouse, sheep, cow, pig and chicken, thesewere present largely as phosphatidylcholines, in contrast 66% of the

    total phospholipid in Homo sapienswas sphingomyelin, with the most abundant being dihydrosphingomyelins,

    in particular SM(d18:0/16:0) and SM(d18:0/24:1). The abundant glycerophospholipids within human lenses

    were found to be predominantly phosphatidylethanolamines and phosphatidylserines with surprisingly high

    concentrations of ether-linked alkyl chains identified in both classes. This study is the first to identify the

    phospholipid class (head-group) and assign the constituent fatty acid(s) for each lipid molecule and to quantify

    individual lens phospholipids using internal standards. These data clearly indicate marked differences in the

    membrane lipid composition of the human lens compared to commonly used animal models and thus predict a

    significant variation in the membrane properties of human lens fibre cells compared to those of other animals.

UOW Authors


  •   Deeley, Jane M. (external author)
  •   Mitchell, Todd
  •   Wei, Xiaojia Eric. (external author)
  •   Korth, John (external author)
  •   Nealon, Jessica
  •   Blanksby, Stephen J. (external author)
  •   Truscott, Roger

Publication Date


  • 2008

Citation


  • Deeley, J. M., Mitchell, T. W., Wei, X., Korth, J., Hughes, J. R., Blanksby, S. J. & Truscott, R. J.W. (2008). Human lens lipids differ markedly from those of commonly used experimental animals. BBA - Molecular and Cell Biology of Lipids, 1781 (6-7), 288-298.

Scopus Eid


  • 2-s2.0-48749119985

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3077&context=hbspapers

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/2024

Number Of Pages


  • 10

Start Page


  • 288

End Page


  • 298

Volume


  • 1781

Issue


  • 6-7

Place Of Publication


  • http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%236183%232008%23982189993%23692912%23FLA%23&_cdi=6183&_pubType=J&_auth=y&_acct=C000014118&_version=1&_urlVersion=0&_userid=202616&md5=22a456dd39170baba69ed19f18d2ff89