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Using calibration approaches to compensate for remaining matrix effects in quantitative liquid chromatography/electrospray ionization multistage mass spectrometric analysis of phytoestrogens in aqueous environmental samples

Journal Article


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Abstract


  • Signal suppression is a common problem in quantitative liquid chromatography/electrospray

    ionization multistage mass spectrometric (LC/ESI-MSn) analysis in environment samples, especially

    in highly loaded wastewater samples with highly complex matrix. Optimization of sample preparation

    and improvement of chromatographic separation are prerequisite to improve reproducibility

    and selectivity. Matrix components are reduced if not eliminated by optimization of sample

    preparation steps. However, extensive sample preparation may be time-consuming and risk the

    significant loss of some trace analytes. The best way to further compensate matrix effects is the use of

    an internal standard for each analyte. However, in a multi-component analysis, finding appropriate

    internal standards for every analyte is often difficult. In this present study, a more practical

    alternative option was sought. Matrix effects were assessed using the post-extraction addition

    method. By comparison of three different calibration approaches, it was found that matrix-matched

    calibration combined with one internal standard provides a satisfactory method for compensating for

    any residual matrix effects on all the analytes. Validating experiments on different sewage treatment

    plant (STP) influent samples analyzing for a range of phytoestrogens showed that this calibration

    method provided satisfactory results with concentration ratio 96.1105.7% compared to those by

    standard addition.

Authors


  •   Kang, Jinguo (external author)
  •   Hick, Larry A. (external author)
  •   Price, William E.

Publication Date


  • 2007

Citation


  • Kang, J., Hick, L. A. & Price, W. E. (2007). Using calibration approaches to compensate for remaining matrix effects in quantitative liquid chromatography/electrospray ionization multistage mass spectrometric analysis of phytoestrogens in aqueous environmental samples. Rapid Communications in Mass Spectrometry, 21 (24), 4065-4072.

Scopus Eid


  • 2-s2.0-37649001812

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2174&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/1105

Number Of Pages


  • 7

Start Page


  • 4065

End Page


  • 4072

Volume


  • 21

Issue


  • 24

Abstract


  • Signal suppression is a common problem in quantitative liquid chromatography/electrospray

    ionization multistage mass spectrometric (LC/ESI-MSn) analysis in environment samples, especially

    in highly loaded wastewater samples with highly complex matrix. Optimization of sample preparation

    and improvement of chromatographic separation are prerequisite to improve reproducibility

    and selectivity. Matrix components are reduced if not eliminated by optimization of sample

    preparation steps. However, extensive sample preparation may be time-consuming and risk the

    significant loss of some trace analytes. The best way to further compensate matrix effects is the use of

    an internal standard for each analyte. However, in a multi-component analysis, finding appropriate

    internal standards for every analyte is often difficult. In this present study, a more practical

    alternative option was sought. Matrix effects were assessed using the post-extraction addition

    method. By comparison of three different calibration approaches, it was found that matrix-matched

    calibration combined with one internal standard provides a satisfactory method for compensating for

    any residual matrix effects on all the analytes. Validating experiments on different sewage treatment

    plant (STP) influent samples analyzing for a range of phytoestrogens showed that this calibration

    method provided satisfactory results with concentration ratio 96.1105.7% compared to those by

    standard addition.

Authors


  •   Kang, Jinguo (external author)
  •   Hick, Larry A. (external author)
  •   Price, William E.

Publication Date


  • 2007

Citation


  • Kang, J., Hick, L. A. & Price, W. E. (2007). Using calibration approaches to compensate for remaining matrix effects in quantitative liquid chromatography/electrospray ionization multistage mass spectrometric analysis of phytoestrogens in aqueous environmental samples. Rapid Communications in Mass Spectrometry, 21 (24), 4065-4072.

Scopus Eid


  • 2-s2.0-37649001812

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2174&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/1105

Number Of Pages


  • 7

Start Page


  • 4065

End Page


  • 4072

Volume


  • 21

Issue


  • 24