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Differential expression of hypothalamic CART mRNA in response to body weight change following different dietary interventions

Journal Article


Abstract


  • Cocaine- and amphetamine-regulated transcript (CART) peptide is widely expressed in the

    hypothalamus and is involved in the central regulation of energy balance. Using in situ hybridization,

    this study examined the roles of CART peptide in the hypothalamus of diet-induced obese (DIO) or dietresistant

    (DR) mice under different dietary interventions including high-fat (HF), low-fat (LF) and pairfeeding

    (PF) diet for 6 weeks. Pair feeding the energy intake of the DIO and DR mice was used to determine

    whether there is an inherent difference in baseline CART expression that may cause the DIO and DR

    phenotypes. The results demonstrated that CART mRNA expression in the hypothalamus of the DIOmice

    responded differently on the high-fat diet compared to DR mice. The arcuate nucleus and paraventricular

    nucleus showed a significant reduction in CART mRNA expression in DIO mice compared to DR mice on

    the HF diet (19.6%, p = 0.019; 26.1%, p = 0.003); whilst a profound increase in CART mRNA expression

    was observed in the dorsomedial nucleus and lateral hypothalamic area (+44.5%, p = 0.007; +37.4%,

    p = 0.033). Our study suggests that the decrease of CARTmRNA expression in Arc and PVN regions of DIO

    mice may contribute to the development of high-fat diet-induced obesity. In addition, CART in the

    dorsomedial nucleus (DM) of hypothalamus and lateral hypothalamus (LH) may be involved in the

    activation of an orexigenic effect. Since pair feeding of the high-fat diet eliminated both the body weight

    and CART mRNA differences between the DIO and DR mice, it is likely that their alterations in gene

    expression were a consequence of their dissimilar body weight levels.

Authors


  •   Yu, Yinghua
  •   South, Timothy (external author)
  •   Wang, Dennis (external author)
  •   Huang, Xu-Feng

Publication Date


  • 2008

Citation


  • Yu, Y., South, T., Wang, Q. & Huang, X. (2008). Differential expression of hypothalamic CART mRNA in response to body weight change following different dietary interventions. Neurochemistry International: the journal for the publication of cellular and molecular aspects of neurochemistry, 52 (8), 1422-1430.

Scopus Eid


  • 2-s2.0-44749087828

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/1935

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 1422

End Page


  • 1430

Volume


  • 52

Issue


  • 8

Place Of Publication


  • United Kingdom

Abstract


  • Cocaine- and amphetamine-regulated transcript (CART) peptide is widely expressed in the

    hypothalamus and is involved in the central regulation of energy balance. Using in situ hybridization,

    this study examined the roles of CART peptide in the hypothalamus of diet-induced obese (DIO) or dietresistant

    (DR) mice under different dietary interventions including high-fat (HF), low-fat (LF) and pairfeeding

    (PF) diet for 6 weeks. Pair feeding the energy intake of the DIO and DR mice was used to determine

    whether there is an inherent difference in baseline CART expression that may cause the DIO and DR

    phenotypes. The results demonstrated that CART mRNA expression in the hypothalamus of the DIOmice

    responded differently on the high-fat diet compared to DR mice. The arcuate nucleus and paraventricular

    nucleus showed a significant reduction in CART mRNA expression in DIO mice compared to DR mice on

    the HF diet (19.6%, p = 0.019; 26.1%, p = 0.003); whilst a profound increase in CART mRNA expression

    was observed in the dorsomedial nucleus and lateral hypothalamic area (+44.5%, p = 0.007; +37.4%,

    p = 0.033). Our study suggests that the decrease of CARTmRNA expression in Arc and PVN regions of DIO

    mice may contribute to the development of high-fat diet-induced obesity. In addition, CART in the

    dorsomedial nucleus (DM) of hypothalamus and lateral hypothalamus (LH) may be involved in the

    activation of an orexigenic effect. Since pair feeding of the high-fat diet eliminated both the body weight

    and CART mRNA differences between the DIO and DR mice, it is likely that their alterations in gene

    expression were a consequence of their dissimilar body weight levels.

Authors


  •   Yu, Yinghua
  •   South, Timothy (external author)
  •   Wang, Dennis (external author)
  •   Huang, Xu-Feng

Publication Date


  • 2008

Citation


  • Yu, Y., South, T., Wang, Q. & Huang, X. (2008). Differential expression of hypothalamic CART mRNA in response to body weight change following different dietary interventions. Neurochemistry International: the journal for the publication of cellular and molecular aspects of neurochemistry, 52 (8), 1422-1430.

Scopus Eid


  • 2-s2.0-44749087828

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/1935

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 1422

End Page


  • 1430

Volume


  • 52

Issue


  • 8

Place Of Publication


  • United Kingdom