Skip to main content
placeholder image

A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling

Journal Article


Abstract


  • The study of neurodegenerative diseases using pluripotent stem cells requires new methods to assess neurodevelopment and neurodegeneration of specific neuronal subtypes. The cholinergic system, characterized by its use of the neurotransmitter acetylcholine, is one of the first to degenerate in Alzheimer's disease and is also affected in frontotemporal dementia. We developed a differentiation protocol to generate basal forebrain-like cholinergic neurons (BFCNs) from induced pluripotent stem cells (iPSCs) aided by the use of small molecule inhibitors and growth factors. Ten iPSC lines were successfully differentiated into BFCNs using this protocol. The neuronal cultures were characterised through RNA and protein expression, and functional analysis of neurons was confirmed by whole-cell patch clamp. We have developed a reliable protocol using only small molecule inhibitors and growth factors, while avoiding transfection or cell sorting methods, to achieve a BFCN culture that expresses the characteristic markers of cholinergic neurons.

Authors


  •   Sanz Munoz, Sonia (external author)
  •   Engel, Martin
  •   Balez, Rachelle (external author)
  •   Do-Ha, Dzung (external author)
  •   Castro Cabral-da-Silva, Mauricio
  •   Hernandez, Damian (external author)
  •   Berg, Tracey (external author)
  •   Fifita, Jennifer (external author)
  •   Grima, Natalie (external author)
  •   Yang, Shu (external author)
  •   Blair, Ian (external author)
  •   Nicholson, Garth (external author)
  •   Cook, Anthony (external author)
  •   Hewitt, Alex (external author)
  •   Pebay, Alice (external author)
  •   Ooi, Lezanne

Publication Date


  • 2020

Published In


Citation


  • Sanz Munoz, S., Engel, M., Balez, R., Do-Ha, P., E Castro Cabral Da Silva, M., Hernandez, D., Berg, T., Fifita, J., Grima, N., Yang, S., Blair, I., Nicholson, G., Cook, A., Hewitt, A., Pebay, A. & Ooi, L. (2020). A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling. Cells, 9 (9),

Scopus Eid


  • 2-s2.0-85090505293

Volume


  • 9

Issue


  • 9

Place Of Publication


  • Switzerland

Abstract


  • The study of neurodegenerative diseases using pluripotent stem cells requires new methods to assess neurodevelopment and neurodegeneration of specific neuronal subtypes. The cholinergic system, characterized by its use of the neurotransmitter acetylcholine, is one of the first to degenerate in Alzheimer's disease and is also affected in frontotemporal dementia. We developed a differentiation protocol to generate basal forebrain-like cholinergic neurons (BFCNs) from induced pluripotent stem cells (iPSCs) aided by the use of small molecule inhibitors and growth factors. Ten iPSC lines were successfully differentiated into BFCNs using this protocol. The neuronal cultures were characterised through RNA and protein expression, and functional analysis of neurons was confirmed by whole-cell patch clamp. We have developed a reliable protocol using only small molecule inhibitors and growth factors, while avoiding transfection or cell sorting methods, to achieve a BFCN culture that expresses the characteristic markers of cholinergic neurons.

Authors


  •   Sanz Munoz, Sonia (external author)
  •   Engel, Martin
  •   Balez, Rachelle (external author)
  •   Do-Ha, Dzung (external author)
  •   Castro Cabral-da-Silva, Mauricio
  •   Hernandez, Damian (external author)
  •   Berg, Tracey (external author)
  •   Fifita, Jennifer (external author)
  •   Grima, Natalie (external author)
  •   Yang, Shu (external author)
  •   Blair, Ian (external author)
  •   Nicholson, Garth (external author)
  •   Cook, Anthony (external author)
  •   Hewitt, Alex (external author)
  •   Pebay, Alice (external author)
  •   Ooi, Lezanne

Publication Date


  • 2020

Published In


Citation


  • Sanz Munoz, S., Engel, M., Balez, R., Do-Ha, P., E Castro Cabral Da Silva, M., Hernandez, D., Berg, T., Fifita, J., Grima, N., Yang, S., Blair, I., Nicholson, G., Cook, A., Hewitt, A., Pebay, A. & Ooi, L. (2020). A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling. Cells, 9 (9),

Scopus Eid


  • 2-s2.0-85090505293

Volume


  • 9

Issue


  • 9

Place Of Publication


  • Switzerland