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Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells

Journal Article


Abstract


  • Glycans, also known as carbohydrates, are abundant upon cell surfaces, where they often mediate host-pathogen interactions. The specific recognition of host glycans by pathogenic lectins is an important process that allows the adherence of bacteria to the host epithelial surface in many species, including Group A Streptococcus (GAS). Glycan microarrays present a sensitive, high-throughput approach for identifying novel lectin-glycan interactions and can be applied in the context of whole bacteria or purified bacterial proteins.

Authors


  •   Indraratna, Anuk (external author)
  •   De Oliveira, David (external author)
  •   Hartley-Tassell, Lauren (external author)
  •   Day, Christopher (external author)
  •   Walker, Mark (external author)
  •   Jennings, Michael (external author)
  •   Sanderson-Smith, Martina L.

Publication Date


  • 2020

Citation


  • Indraratna, A., De Oliveira, D., Hartley-Tassell, L., Day, C., Walker, M., Jennings, M. & Sanderson-Smith, M. (2020). Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells. Methods in Molecular Biology, 2136 145-151.

Scopus Eid


  • 2-s2.0-85085157767

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers1/1352

Number Of Pages


  • 6

Start Page


  • 145

End Page


  • 151

Volume


  • 2136

Place Of Publication


  • United States

Abstract


  • Glycans, also known as carbohydrates, are abundant upon cell surfaces, where they often mediate host-pathogen interactions. The specific recognition of host glycans by pathogenic lectins is an important process that allows the adherence of bacteria to the host epithelial surface in many species, including Group A Streptococcus (GAS). Glycan microarrays present a sensitive, high-throughput approach for identifying novel lectin-glycan interactions and can be applied in the context of whole bacteria or purified bacterial proteins.

Authors


  •   Indraratna, Anuk (external author)
  •   De Oliveira, David (external author)
  •   Hartley-Tassell, Lauren (external author)
  •   Day, Christopher (external author)
  •   Walker, Mark (external author)
  •   Jennings, Michael (external author)
  •   Sanderson-Smith, Martina L.

Publication Date


  • 2020

Citation


  • Indraratna, A., De Oliveira, D., Hartley-Tassell, L., Day, C., Walker, M., Jennings, M. & Sanderson-Smith, M. (2020). Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells. Methods in Molecular Biology, 2136 145-151.

Scopus Eid


  • 2-s2.0-85085157767

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers1/1352

Number Of Pages


  • 6

Start Page


  • 145

End Page


  • 151

Volume


  • 2136

Place Of Publication


  • United States