Skip to main content
placeholder image

Fabrication of Aligned Biomimetic Gellan Gum-Chitosan Microstructures through 3D Printed Microfluidic Channels and Multiple In Situ Cross-Linking Mechanisms

Journal Article


Download full-text (Open Access)

Abstract


  • In this study we use a combination of ionic- and photo-cross-linking to develop a fabrication method for producing biocompatible microstructures using a methacrylated gellan gum (a polyanion) and chitosan (a polycation) in addition to lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as the photoinitiator. This work involves the development of a low-cost, portable 3D bioprinter and a customized extrusion mechanism for controlled introduction of the materials through a 3D printed microfluidic nozzle, before being cross-linked in situ to form robust microstructure bundles. The formed microstructures yielded a diameter of less than 1 μm and a tensile strength range of ∼1 MPa. This study is the first to explore and achieve GGMA:CHT microstructure fabrication by means of controlled in-line compaction and photo-cross-linking through 3D printed microfluidic channels.

Publication Date


  • 2020

Citation


  • Robinson, T. M., Talebian, S., Foroughi, J., Yue, Z., Fay, C. D. & Wallace, G. G. (2020). Fabrication of Aligned Biomimetic Gellan Gum-Chitosan Microstructures through 3D Printed Microfluidic Channels and Multiple In Situ Cross-Linking Mechanisms. ACS Biomaterials Science and Engineering, Online First A-K.

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=1330&context=smartpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smartpapers/303

Start Page


  • A

End Page


  • K

Volume


  • Online First

Place Of Publication


  • United States

Abstract


  • In this study we use a combination of ionic- and photo-cross-linking to develop a fabrication method for producing biocompatible microstructures using a methacrylated gellan gum (a polyanion) and chitosan (a polycation) in addition to lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as the photoinitiator. This work involves the development of a low-cost, portable 3D bioprinter and a customized extrusion mechanism for controlled introduction of the materials through a 3D printed microfluidic nozzle, before being cross-linked in situ to form robust microstructure bundles. The formed microstructures yielded a diameter of less than 1 μm and a tensile strength range of ∼1 MPa. This study is the first to explore and achieve GGMA:CHT microstructure fabrication by means of controlled in-line compaction and photo-cross-linking through 3D printed microfluidic channels.

Publication Date


  • 2020

Citation


  • Robinson, T. M., Talebian, S., Foroughi, J., Yue, Z., Fay, C. D. & Wallace, G. G. (2020). Fabrication of Aligned Biomimetic Gellan Gum-Chitosan Microstructures through 3D Printed Microfluidic Channels and Multiple In Situ Cross-Linking Mechanisms. ACS Biomaterials Science and Engineering, Online First A-K.

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=1330&context=smartpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smartpapers/303

Start Page


  • A

End Page


  • K

Volume


  • Online First

Place Of Publication


  • United States