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Fabrication of Aligned Biomimetic Gellan Gum-Chitosan Microstructures through 3D Printed Microfluidic Channels and Multiple In Situ Cross-Linking Mechanisms

Journal Article


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Abstract


  • In this study we use a combination of ionic- and photo-cross-linking to develop a fabrication method for producing biocompatible microstructures using a methacrylated gellan gum (a polyanion) and chitosan (a polycation) in addition to lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as the photoinitiator. This work involves the development of a low-cost, portable 3D bioprinter and a customized extrusion mechanism for controlled introduction of the materials through a 3D printed microfluidic nozzle, before being cross-linked in situ to form robust microstructure bundles. The formed microstructures yielded a diameter of less than 1 μm and a tensile strength range of ∼1 MPa. This study is the first to explore and achieve GGMA:CHT microstructure fabrication by means of controlled in-line compaction and photo-cross-linking through 3D printed microfluidic channels.

Publication Date


  • 2020

Citation


  • Robinson, T. M., Talebian, S., Foroughi, J., Yue, Z., Fay, C. D. & Wallace, G. G. (2020). Fabrication of Aligned Biomimetic Gellan Gum-Chitosan Microstructures through 3D Printed Microfluidic Channels and Multiple In Situ Cross-Linking Mechanisms. ACS Biomaterials Science and Engineering, 6 (6), 3638-3648.

Scopus Eid


  • 2-s2.0-85090394813

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=1330&context=smartpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smartpapers/303

Number Of Pages


  • 10

Start Page


  • 3638

End Page


  • 3648

Volume


  • 6

Issue


  • 6

Place Of Publication


  • United States

Abstract


  • In this study we use a combination of ionic- and photo-cross-linking to develop a fabrication method for producing biocompatible microstructures using a methacrylated gellan gum (a polyanion) and chitosan (a polycation) in addition to lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as the photoinitiator. This work involves the development of a low-cost, portable 3D bioprinter and a customized extrusion mechanism for controlled introduction of the materials through a 3D printed microfluidic nozzle, before being cross-linked in situ to form robust microstructure bundles. The formed microstructures yielded a diameter of less than 1 μm and a tensile strength range of ∼1 MPa. This study is the first to explore and achieve GGMA:CHT microstructure fabrication by means of controlled in-line compaction and photo-cross-linking through 3D printed microfluidic channels.

Publication Date


  • 2020

Citation


  • Robinson, T. M., Talebian, S., Foroughi, J., Yue, Z., Fay, C. D. & Wallace, G. G. (2020). Fabrication of Aligned Biomimetic Gellan Gum-Chitosan Microstructures through 3D Printed Microfluidic Channels and Multiple In Situ Cross-Linking Mechanisms. ACS Biomaterials Science and Engineering, 6 (6), 3638-3648.

Scopus Eid


  • 2-s2.0-85090394813

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=1330&context=smartpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smartpapers/303

Number Of Pages


  • 10

Start Page


  • 3638

End Page


  • 3648

Volume


  • 6

Issue


  • 6

Place Of Publication


  • United States