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Bioprinting Stem Cells in Hydrogel for In Situ Surgical Application: A Case for Articular Cartilage

Journal Article


Abstract


  • Three-dimensional (3D) bioprinting is driving major innovations in the area of cartilage tissue engineering. As an alternative to computer-aided 3D printing, in situ additive manufacturing has the advantage of matching the geometry of the defect to be repaired without specific preliminary image analysis, shaping the bioscaffold within the defect, and achieving the best possible contact between the bioscaffold and the host tissue. Here, we describe an in situ approach that allows 3D bioprinting of human adipose-derived stem cells (hADSCs) laden in 10%GelMa/2%HAMa (GelMa/HAMa) hydrogel. We use coaxial extrusion to obtain a core/shell bioscaffold with high cell viability, as well as adequate mechanical properties for articular cartilage regeneration and repair.

Authors


  •   Duchi, Serena (external author)
  •   Onofrillo, Carmine (external author)
  •   O'Connell, Cathal (external author)
  •   Wallace, Gordon G.
  •   Choong, Peter (external author)
  •   Di Bella, Claudia (external author)

Publication Date


  • 2020

Citation


  • Duchi, S., Onofrillo, C., O'Connell, C., Wallace, G., Choong, P. & Di Bella, C. (2020). Bioprinting Stem Cells in Hydrogel for In Situ Surgical Application: A Case for Articular Cartilage. Methods in Molecular Biology, 2140 145-157.

Scopus Eid


  • 2-s2.0-85082259290

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/4075

Number Of Pages


  • 12

Start Page


  • 145

End Page


  • 157

Volume


  • 2140

Place Of Publication


  • United States

Abstract


  • Three-dimensional (3D) bioprinting is driving major innovations in the area of cartilage tissue engineering. As an alternative to computer-aided 3D printing, in situ additive manufacturing has the advantage of matching the geometry of the defect to be repaired without specific preliminary image analysis, shaping the bioscaffold within the defect, and achieving the best possible contact between the bioscaffold and the host tissue. Here, we describe an in situ approach that allows 3D bioprinting of human adipose-derived stem cells (hADSCs) laden in 10%GelMa/2%HAMa (GelMa/HAMa) hydrogel. We use coaxial extrusion to obtain a core/shell bioscaffold with high cell viability, as well as adequate mechanical properties for articular cartilage regeneration and repair.

Authors


  •   Duchi, Serena (external author)
  •   Onofrillo, Carmine (external author)
  •   O'Connell, Cathal (external author)
  •   Wallace, Gordon G.
  •   Choong, Peter (external author)
  •   Di Bella, Claudia (external author)

Publication Date


  • 2020

Citation


  • Duchi, S., Onofrillo, C., O'Connell, C., Wallace, G., Choong, P. & Di Bella, C. (2020). Bioprinting Stem Cells in Hydrogel for In Situ Surgical Application: A Case for Articular Cartilage. Methods in Molecular Biology, 2140 145-157.

Scopus Eid


  • 2-s2.0-85082259290

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/4075

Number Of Pages


  • 12

Start Page


  • 145

End Page


  • 157

Volume


  • 2140

Place Of Publication


  • United States