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The mRNA-based reprogramming of fibroblasts from a SOD1E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007

Journal Article


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Abstract


  • © 2020 The Authors Dermal fibroblasts were donated by a 43 year old male patient with clinically diagnosed familial amyotrophic lateral sclerosis (ALS), carrying the SOD1E101G mutation. The induced pluripotent stem cell (iPSC) line UOWi007-A was generated using repeated mRNA transfections for pluripotency transcription factors Oct4, Klf4, Sox2, c-Myc, Lin28 and Nanog. The iPSCs carried the SOD1E101G genotype and had a normal karyotype, expressed expected pluripotency markers and were capable of in vitro differentiation into endodermal, mesodermal and ectodermal lineages. This iPSC line may be useful for investigating familial ALS resulting from a SOD1 E101G mutation.

Authors


  •   Balez, Rachelle (external author)
  •   Berg, Tracey (external author)
  •   Bax, Monique (external author)
  •   Sanz Munoz, Sonia (external author)
  •   Castro Cabral-da-Silva, Mauricio
  •   Engel, Martin
  •   Do-Ha, Phuong Dzung (external author)
  •   Stevens, Claire H.
  •   Rowe, Dominic (external author)
  •   Yang, Shu (external author)
  •   Blair, Ian (external author)
  •   Ooi, Lezanne

Publication Date


  • 2020

Citation


  • Balez, R., Berg, T., Bax, M., Sanz Munoz, S., E Castro Cabral Da Silva, M., Engel, M., Do-Ha, P., Stevens, C., Rowe, D., Yang, S., Blair, I. & Ooi, L. (2020). The mRNA-based reprogramming of fibroblasts from a SOD1E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007. Stem Cell Research, 42

Scopus Eid


  • 2-s2.0-85078314408

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=2154&context=smhpapers1

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers1/1140

Volume


  • 42

Place Of Publication


  • Netherlands

Abstract


  • © 2020 The Authors Dermal fibroblasts were donated by a 43 year old male patient with clinically diagnosed familial amyotrophic lateral sclerosis (ALS), carrying the SOD1E101G mutation. The induced pluripotent stem cell (iPSC) line UOWi007-A was generated using repeated mRNA transfections for pluripotency transcription factors Oct4, Klf4, Sox2, c-Myc, Lin28 and Nanog. The iPSCs carried the SOD1E101G genotype and had a normal karyotype, expressed expected pluripotency markers and were capable of in vitro differentiation into endodermal, mesodermal and ectodermal lineages. This iPSC line may be useful for investigating familial ALS resulting from a SOD1 E101G mutation.

Authors


  •   Balez, Rachelle (external author)
  •   Berg, Tracey (external author)
  •   Bax, Monique (external author)
  •   Sanz Munoz, Sonia (external author)
  •   Castro Cabral-da-Silva, Mauricio
  •   Engel, Martin
  •   Do-Ha, Phuong Dzung (external author)
  •   Stevens, Claire H.
  •   Rowe, Dominic (external author)
  •   Yang, Shu (external author)
  •   Blair, Ian (external author)
  •   Ooi, Lezanne

Publication Date


  • 2020

Citation


  • Balez, R., Berg, T., Bax, M., Sanz Munoz, S., E Castro Cabral Da Silva, M., Engel, M., Do-Ha, P., Stevens, C., Rowe, D., Yang, S., Blair, I. & Ooi, L. (2020). The mRNA-based reprogramming of fibroblasts from a SOD1E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007. Stem Cell Research, 42

Scopus Eid


  • 2-s2.0-85078314408

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=2154&context=smhpapers1

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers1/1140

Volume


  • 42

Place Of Publication


  • Netherlands