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Spy-ing on Cas9: Single-molecule tools reveal the enzymology of Cas9

Journal Article


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Abstract


  • Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) systems are an adaptive immune response mechanism in prokaryotes which can target and cleave invading DNA or RNA. The rapid understanding of the type II CRISPR/Cas9 system through biochemical, genetic and structural investigations has contributed to the development of Cas9 for various DNA- and RNA-targeting applications. Recent single-molecule investigations of CRISPR/Cas9 mechanisms have further extended our understanding of target search, binding and cleavage. These investigations are fundamental to the further development of CRISPR/Cas9 tools. This review discusses how single-molecule techniques have illuminated the enzymology of Cas9 endonucleases.

Publication Date


  • 2019

Citation


  • Whinn, K. S., van Oijen, A. M. & Ghodke, H. (2019). Spy-ing on Cas9: Single-molecule tools reveal the enzymology of Cas9. Current Opinion in Biomedical Engineering, 12 25-33.

Scopus Eid


  • 2-s2.0-85073918042

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=1990&context=smhpapers1

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers1/976

Number Of Pages


  • 8

Start Page


  • 25

End Page


  • 33

Volume


  • 12

Place Of Publication


  • United States

Abstract


  • Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) systems are an adaptive immune response mechanism in prokaryotes which can target and cleave invading DNA or RNA. The rapid understanding of the type II CRISPR/Cas9 system through biochemical, genetic and structural investigations has contributed to the development of Cas9 for various DNA- and RNA-targeting applications. Recent single-molecule investigations of CRISPR/Cas9 mechanisms have further extended our understanding of target search, binding and cleavage. These investigations are fundamental to the further development of CRISPR/Cas9 tools. This review discusses how single-molecule techniques have illuminated the enzymology of Cas9 endonucleases.

Publication Date


  • 2019

Citation


  • Whinn, K. S., van Oijen, A. M. & Ghodke, H. (2019). Spy-ing on Cas9: Single-molecule tools reveal the enzymology of Cas9. Current Opinion in Biomedical Engineering, 12 25-33.

Scopus Eid


  • 2-s2.0-85073918042

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=1990&context=smhpapers1

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers1/976

Number Of Pages


  • 8

Start Page


  • 25

End Page


  • 33

Volume


  • 12

Place Of Publication


  • United States