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Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells

Journal Article


Abstract


  • Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection. We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures. Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression. Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile. Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo. These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.

Authors


  •   De Oliveira, David (external author)
  •   Everest-Dass, Arun (external author)
  •   Hartley-Tassell, Lauren (external author)
  •   Day, Christopher (external author)
  •   Indraratna, Anuk (external author)
  •   Brouwer, Stephan (external author)
  •   Cleary, Ailish (external author)
  •   Kautto, Liisa (external author)
  •   Gorman, Jody L. (external author)
  •   Packer, Nicolle (external author)
  •   Jennings, Michael (external author)
  •   Walker, Mark J. (external author)
  •   Sanderson-Smith, Martina L.

Publication Date


  • 2019

Citation


  • De Oliveira, D. M.P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J. & Sanderson-Smith, M. L. (2019). Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells. The FASEB Journal, 33 (10), 10808-10818.

Scopus Eid


  • 2-s2.0-85072716495

Number Of Pages


  • 10

Start Page


  • 10808

End Page


  • 10818

Volume


  • 33

Issue


  • 10

Place Of Publication


  • United States

Abstract


  • Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection. We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures. Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression. Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile. Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo. These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.

Authors


  •   De Oliveira, David (external author)
  •   Everest-Dass, Arun (external author)
  •   Hartley-Tassell, Lauren (external author)
  •   Day, Christopher (external author)
  •   Indraratna, Anuk (external author)
  •   Brouwer, Stephan (external author)
  •   Cleary, Ailish (external author)
  •   Kautto, Liisa (external author)
  •   Gorman, Jody L. (external author)
  •   Packer, Nicolle (external author)
  •   Jennings, Michael (external author)
  •   Walker, Mark J. (external author)
  •   Sanderson-Smith, Martina L.

Publication Date


  • 2019

Citation


  • De Oliveira, D. M.P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J. & Sanderson-Smith, M. L. (2019). Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells. The FASEB Journal, 33 (10), 10808-10818.

Scopus Eid


  • 2-s2.0-85072716495

Number Of Pages


  • 10

Start Page


  • 10808

End Page


  • 10818

Volume


  • 33

Issue


  • 10

Place Of Publication


  • United States