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Identification of Multiple Kinetic Populations of DNA-Binding Proteins in Live Cells

Journal Article


Abstract


  • Understanding how multiprotein complexes function in cells requires detailed quantitative understanding of their association and dissociation kinetics. Analysis of the heterogeneity of binding lifetimes enables the interrogation of the various intermediate states formed during the reaction. Single-molecule fluorescence imaging permits the measurement of reaction kinetics inside living organisms with minimal perturbation. However, poor photophysical properties of fluorescent probes limit the dynamic range and accuracy of measurements of off rates in live cells. Time-lapse single-molecule fluorescence imaging can partially overcome the limits of photobleaching; however, limitations of this technique remain uncharacterized. Here, we present a structured analysis of which timescales are most accessible using the time-lapse imaging approach and explore uncertainties in determining kinetic subpopulations. We demonstrate the effect of shot noise on the precision of the measurements as well as the resolution and dynamic range limits that are inherent to the method. Our work provides a convenient implementation to determine theoretical errors from measurements and to support interpretation of experimental data.

Publication Date


  • 2019

Citation


  • Ho, H. N., Zalami, D., Kohler, J., van Oijen, A. M. & Ghodke, H. (2019). Identification of Multiple Kinetic Populations of DNA-Binding Proteins in Live Cells. Biophysical Journal, 117 (5), 950-961.

Scopus Eid


  • 2-s2.0-85071707562

Number Of Pages


  • 11

Start Page


  • 950

End Page


  • 961

Volume


  • 117

Issue


  • 5

Place Of Publication


  • United States

Abstract


  • Understanding how multiprotein complexes function in cells requires detailed quantitative understanding of their association and dissociation kinetics. Analysis of the heterogeneity of binding lifetimes enables the interrogation of the various intermediate states formed during the reaction. Single-molecule fluorescence imaging permits the measurement of reaction kinetics inside living organisms with minimal perturbation. However, poor photophysical properties of fluorescent probes limit the dynamic range and accuracy of measurements of off rates in live cells. Time-lapse single-molecule fluorescence imaging can partially overcome the limits of photobleaching; however, limitations of this technique remain uncharacterized. Here, we present a structured analysis of which timescales are most accessible using the time-lapse imaging approach and explore uncertainties in determining kinetic subpopulations. We demonstrate the effect of shot noise on the precision of the measurements as well as the resolution and dynamic range limits that are inherent to the method. Our work provides a convenient implementation to determine theoretical errors from measurements and to support interpretation of experimental data.

Publication Date


  • 2019

Citation


  • Ho, H. N., Zalami, D., Kohler, J., van Oijen, A. M. & Ghodke, H. (2019). Identification of Multiple Kinetic Populations of DNA-Binding Proteins in Live Cells. Biophysical Journal, 117 (5), 950-961.

Scopus Eid


  • 2-s2.0-85071707562

Number Of Pages


  • 11

Start Page


  • 950

End Page


  • 961

Volume


  • 117

Issue


  • 5

Place Of Publication


  • United States