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Sheathless separation of microalgae from bacteria using a simple straight channel based on viscoelastic microfluidics

Journal Article


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Abstract


  • Microalgae cells have been recognized as a promising sustainable resource to meet worldwide growing demands for renewable energy, food, livestock feed, water, cosmetics, pharmaceuticals, and materials. In order to ensure high-efficiency and high-quality production of biomass, biofuel, or bio-based products, purification procedures prior to the storage and cultivation of the microalgae from contaminated bacteria are of great importance. The present work proposed and developed a simple, sheathless, and efficient method to separate microalgae Chlorella from bacteria Bacillus Subtilis in a straight channel using the viscoelasticity of the medium. Microalgae and bacteria migrate to different lateral positions closer to the channel centre and channel walls respectively. Fluorescent microparticles with 1 μm and 5 μm diameters were first used to mimic the behaviours of bacteria and microalgae to optimize the separating conditions. Subsequently, size-based separation in Newtonian fluid and in viscoelastic fluid in straight channels with different aspect ratios was compared and demonstrated. Under the optimal condition, the removal ratio for 1 μm microparticles and separation efficiency for 5 μm particles can reach up to 98.28% and 93.85% respectively. For bacteria and microalgae cells separation, the removal ratio for bacteria and separation efficiency for microalgae cells is 92.69% and 100% respectively. This work demonstrated the continuous and sheathless separation of microalgae from bacteria for the first time by viscoelastic microfluidics. This technique can also be applied as an efficient and user-friendly method to separate mammalian cells or other kinds of cells.

UOW Authors


  •   Yuan, Dan (external author)
  •   Zhao, Qianbin (external author)
  •   Yan, Sheng (external author)
  •   Tang, Shiyang (external author)
  •   Zhang, Yuxin (external author)
  •   Yun, Guolin (external author)
  •   Nguyen, Nam-Trung (external author)
  •   Zhang, Jun (external author)
  •   Li, Ming (external author)
  •   Li, Weihua

Publication Date


  • 2019

Citation


  • Yuan, D., Zhao, Q., Yan, S., Tang, S., Zhang, Y., Yun, G., Nguyen, N., Zhang, J., Li, M. & Li, W. (2019). Sheathless separation of microalgae from bacteria using a simple straight channel based on viscoelastic microfluidics. Lab on a Chip, 19 (17), 2811-2821.

Scopus Eid


  • 2-s2.0-85071191052

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=4152&context=eispapers1

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers1/3133

Number Of Pages


  • 10

Start Page


  • 2811

End Page


  • 2821

Volume


  • 19

Issue


  • 17

Place Of Publication


  • United Kingdom

Abstract


  • Microalgae cells have been recognized as a promising sustainable resource to meet worldwide growing demands for renewable energy, food, livestock feed, water, cosmetics, pharmaceuticals, and materials. In order to ensure high-efficiency and high-quality production of biomass, biofuel, or bio-based products, purification procedures prior to the storage and cultivation of the microalgae from contaminated bacteria are of great importance. The present work proposed and developed a simple, sheathless, and efficient method to separate microalgae Chlorella from bacteria Bacillus Subtilis in a straight channel using the viscoelasticity of the medium. Microalgae and bacteria migrate to different lateral positions closer to the channel centre and channel walls respectively. Fluorescent microparticles with 1 μm and 5 μm diameters were first used to mimic the behaviours of bacteria and microalgae to optimize the separating conditions. Subsequently, size-based separation in Newtonian fluid and in viscoelastic fluid in straight channels with different aspect ratios was compared and demonstrated. Under the optimal condition, the removal ratio for 1 μm microparticles and separation efficiency for 5 μm particles can reach up to 98.28% and 93.85% respectively. For bacteria and microalgae cells separation, the removal ratio for bacteria and separation efficiency for microalgae cells is 92.69% and 100% respectively. This work demonstrated the continuous and sheathless separation of microalgae from bacteria for the first time by viscoelastic microfluidics. This technique can also be applied as an efficient and user-friendly method to separate mammalian cells or other kinds of cells.

UOW Authors


  •   Yuan, Dan (external author)
  •   Zhao, Qianbin (external author)
  •   Yan, Sheng (external author)
  •   Tang, Shiyang (external author)
  •   Zhang, Yuxin (external author)
  •   Yun, Guolin (external author)
  •   Nguyen, Nam-Trung (external author)
  •   Zhang, Jun (external author)
  •   Li, Ming (external author)
  •   Li, Weihua

Publication Date


  • 2019

Citation


  • Yuan, D., Zhao, Q., Yan, S., Tang, S., Zhang, Y., Yun, G., Nguyen, N., Zhang, J., Li, M. & Li, W. (2019). Sheathless separation of microalgae from bacteria using a simple straight channel based on viscoelastic microfluidics. Lab on a Chip, 19 (17), 2811-2821.

Scopus Eid


  • 2-s2.0-85071191052

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=4152&context=eispapers1

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers1/3133

Number Of Pages


  • 10

Start Page


  • 2811

End Page


  • 2821

Volume


  • 19

Issue


  • 17

Place Of Publication


  • United Kingdom