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Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming

Journal Article


Abstract


  • © 2019 The Authors Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.

Authors


  •   Bax, Monique (external author)
  •   Balez, Rachelle (external author)
  •   Sanz Munoz, Sonia (external author)
  •   Do-Ha, Phuong Dzung (external author)
  •   Stevens, Claire H.
  •   Berg, Tracey (external author)
  •   Castro Cabral-da-Silva, Mauricio
  •   Engel, Martin
  •   Nicholson, Garth A. (external author)
  •   Yang, Shu (external author)
  •   Blair, Ian P. (external author)
  •   Ooi, Lezanne

Publication Date


  • 2019

Citation


  • Bax, M., Balez, R., Sanz Munoz, S., Do-Ha, D., Stevens, C. H., Berg, T., Cabral-da-Silva, M. C., Engel, M., Nicholson, G., Yang, S., Blair, I. P. & Ooi, L. (2019). Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming. Stem Cell Research, 40

Scopus Eid


  • 2-s2.0-85070890584

Volume


  • 40

Place Of Publication


  • Netherlands

Abstract


  • © 2019 The Authors Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.

Authors


  •   Bax, Monique (external author)
  •   Balez, Rachelle (external author)
  •   Sanz Munoz, Sonia (external author)
  •   Do-Ha, Phuong Dzung (external author)
  •   Stevens, Claire H.
  •   Berg, Tracey (external author)
  •   Castro Cabral-da-Silva, Mauricio
  •   Engel, Martin
  •   Nicholson, Garth A. (external author)
  •   Yang, Shu (external author)
  •   Blair, Ian P. (external author)
  •   Ooi, Lezanne

Publication Date


  • 2019

Citation


  • Bax, M., Balez, R., Sanz Munoz, S., Do-Ha, D., Stevens, C. H., Berg, T., Cabral-da-Silva, M. C., Engel, M., Nicholson, G., Yang, S., Blair, I. P. & Ooi, L. (2019). Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming. Stem Cell Research, 40

Scopus Eid


  • 2-s2.0-85070890584

Volume


  • 40

Place Of Publication


  • Netherlands