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3D Scaffolds of Polycaprolactone/Copper-Doped Bioactive Glass: Architecture Engineering with Additive Manufacturing and CellularAssessments in a Coculture of Bone Marrow Stem Cells and Endothelial Cells

Journal Article


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Abstract


  • The local delivery of Cu2+ from copper-doped bioactive glass (Cu-BaG) was combined with 3D printing of polycaprolactone (PCL) scaffolds for its potent angiogenic effect in bone tissue engineering. PCL and Cu-BaG were, respectively, dissolved and dispersed in acetone to formulate a moderately homogeneous ink. The PCL/Cu-BaG scaffolds were fabricated via direct ink writing into a cold ethanol bath. The architecture of the printed scaffolds, including strut diameter, strut spacing, and porosity, were investigated and characterized. The PCL/Cu-BaG scaffolds showed a Cu-BaG content-dependent mechanical property, as the compressive Young's modulus ranged from 7 to 13 MPa at an apparent porosity of 60%. The ion dissolution behavior in simulated body fluid was evaluated, and the hydroxyapatite-like precipitation on the strut surface was confirmed. Furthermore, the cytocompatibility of the PCL/Cu-BaG scaffolds was assessed in human bone marrow stem cell (hBMSC) culture, and a dose-dependent cytotoxicity of Cu2+ was observed. Here, the PCL/BaG scaffold induced the higher expression of late osteogenic genes OSTEOCALCIN and DLX5 in comparison to the PCL scaffold. The doping of Cu2+ in BaG elicited higher expression of the early osteogenic marker gene RUNX2a but decreased the expression of late osteogenic marker genes OSTEOCALCIN and DLX5 in comparison to the PCL/BaG scaffold, demonstrating the suppressing effect of Cu2+ on osteogenic differentiation of hBMSCs. In a coculture of hBMSCs and human umbilical vein endothelial cells, both the PCL/BaG and PCL/Cu-BaG scaffolds stimulated the formation of a denser tubule network, compared to the PCL scaffold. Meanwhile, only slightly higher gene expression of vWF was observed with the PCL/Cu-BaG scaffold than with the PCL/BaG scaffold, indicating the potent angiogenic effect of the released Cu2+.

Authors


  •   Wang, Xiaoju (external author)
  •   Zhang, Binbin (external author)
  •   Pitkanen, Sanna (external author)
  •   Ojansivu, Miina (external author)
  •   Xu, Chunlin (external author)
  •   Hannula, Markus (external author)
  •   Hyttinen, Jari A. (external author)
  •   Miettinen, Susanna S. (external author)
  •   Hupa, Leena (external author)
  •   Wallace, Gordon G.

Publication Date


  • 2019

Citation


  • Wang, X., Molino, B. Zhang., Pitkanen, S., Ojansivu, M., Xu, C., Hannula, M., Hyttinen, J., Miettinen, S., Hupa, L. & Wallace, G. (2019). 3D Scaffolds of Polycaprolactone/Copper-Doped Bioactive Glass: Architecture Engineering with Additive Manufacturing and CellularAssessments in a Coculture of Bone Marrow Stem Cells and Endothelial Cells. ACS Biomaterials Science and Engineering, 5 (9), 4496-4510.

Scopus Eid


  • 2-s2.0-85070677224

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=4844&context=aiimpapers

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/3789

Number Of Pages


  • 14

Start Page


  • 4496

End Page


  • 4510

Volume


  • 5

Issue


  • 9

Place Of Publication


  • United States

Abstract


  • The local delivery of Cu2+ from copper-doped bioactive glass (Cu-BaG) was combined with 3D printing of polycaprolactone (PCL) scaffolds for its potent angiogenic effect in bone tissue engineering. PCL and Cu-BaG were, respectively, dissolved and dispersed in acetone to formulate a moderately homogeneous ink. The PCL/Cu-BaG scaffolds were fabricated via direct ink writing into a cold ethanol bath. The architecture of the printed scaffolds, including strut diameter, strut spacing, and porosity, were investigated and characterized. The PCL/Cu-BaG scaffolds showed a Cu-BaG content-dependent mechanical property, as the compressive Young's modulus ranged from 7 to 13 MPa at an apparent porosity of 60%. The ion dissolution behavior in simulated body fluid was evaluated, and the hydroxyapatite-like precipitation on the strut surface was confirmed. Furthermore, the cytocompatibility of the PCL/Cu-BaG scaffolds was assessed in human bone marrow stem cell (hBMSC) culture, and a dose-dependent cytotoxicity of Cu2+ was observed. Here, the PCL/BaG scaffold induced the higher expression of late osteogenic genes OSTEOCALCIN and DLX5 in comparison to the PCL scaffold. The doping of Cu2+ in BaG elicited higher expression of the early osteogenic marker gene RUNX2a but decreased the expression of late osteogenic marker genes OSTEOCALCIN and DLX5 in comparison to the PCL/BaG scaffold, demonstrating the suppressing effect of Cu2+ on osteogenic differentiation of hBMSCs. In a coculture of hBMSCs and human umbilical vein endothelial cells, both the PCL/BaG and PCL/Cu-BaG scaffolds stimulated the formation of a denser tubule network, compared to the PCL scaffold. Meanwhile, only slightly higher gene expression of vWF was observed with the PCL/Cu-BaG scaffold than with the PCL/BaG scaffold, indicating the potent angiogenic effect of the released Cu2+.

Authors


  •   Wang, Xiaoju (external author)
  •   Zhang, Binbin (external author)
  •   Pitkanen, Sanna (external author)
  •   Ojansivu, Miina (external author)
  •   Xu, Chunlin (external author)
  •   Hannula, Markus (external author)
  •   Hyttinen, Jari A. (external author)
  •   Miettinen, Susanna S. (external author)
  •   Hupa, Leena (external author)
  •   Wallace, Gordon G.

Publication Date


  • 2019

Citation


  • Wang, X., Molino, B. Zhang., Pitkanen, S., Ojansivu, M., Xu, C., Hannula, M., Hyttinen, J., Miettinen, S., Hupa, L. & Wallace, G. (2019). 3D Scaffolds of Polycaprolactone/Copper-Doped Bioactive Glass: Architecture Engineering with Additive Manufacturing and CellularAssessments in a Coculture of Bone Marrow Stem Cells and Endothelial Cells. ACS Biomaterials Science and Engineering, 5 (9), 4496-4510.

Scopus Eid


  • 2-s2.0-85070677224

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=4844&context=aiimpapers

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/3789

Number Of Pages


  • 14

Start Page


  • 4496

End Page


  • 4510

Volume


  • 5

Issue


  • 9

Place Of Publication


  • United States