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Effect of acidification on elemental and isotopic compositions of sediment organic matter and macro‐invertebrate muscle tissues in food web research

Journal Article


Abstract


  • Stable isotope techniques in food web studies often focus on organic carbon in food sources which

    are subsequently assimilated in the tissue of consumer organisms through diet. The presence of nondietary

    carbonates in bulk samples can affect their d13C values, altering how their results are

    interpreted. Acidification of samples is a common practice to eliminate any inorganic carbon present

    prior to analysis. We examined the effects of pre-analysis acidification on two size fractions of

    sediment organic matter (SOM) from marine and freshwater wetlands and pure muscle tissue of a

    common freshwater invertebrate (Cherax destructor). The elemental content and isotopic ratios of

    carbon and nitrogen were compared between paired samples of acidified and control treatments. Our

    results showed that acidification does not affect the elemental or isotopic values of freshwater SOM.

    In the marine environment acidification depleted the d13C and d15N values of the fine fraction of

    saltmarsh and d15N values of mangrove fine SOM. Whilst acidification did not change the elemental

    content of invertebrate muscle tissue, the d13C and d15N values were affected. We recommend to

    researchers considering using acidification techniques on material prepared for stable isotope

    analysis that a formal assessment of the effect of acidification on their particular sample type should

    be undertaken. Further detailed investigation to understand the impact of acidification on elemental

    and isotopic values of organic matter and muscular tissues is required.

UOW Authors


  •   Mazumder, Debashish (external author)
  •   Iles, Jordan (external author)
  •   Kelleway, Jeffrey
  •   Kobayashi, Tsuyoshi (external author)
  •   Knowles, Lisa (external author)
  •   Saintilan, Neil (external author)
  •   Hollins, Suzanne E. (external author)

Publication Date


  • 2010

Citation


  • Mazumder, D., Iles, J., Kelleway, J., Kobayashi, T., Knowles, L., Saintilan, N. & Hollins, S. (2010). Effect of acidification on elemental and isotopic compositions of sediment organic matter and macro‐invertebrate muscle tissues in food web research. Rapid Communications in Mass Spectrometry, 24 2938-2942.

Scopus Eid


  • 2-s2.0-77956943196

Number Of Pages


  • 4

Start Page


  • 2938

End Page


  • 2942

Volume


  • 24

Place Of Publication


  • United Kingdom

Abstract


  • Stable isotope techniques in food web studies often focus on organic carbon in food sources which

    are subsequently assimilated in the tissue of consumer organisms through diet. The presence of nondietary

    carbonates in bulk samples can affect their d13C values, altering how their results are

    interpreted. Acidification of samples is a common practice to eliminate any inorganic carbon present

    prior to analysis. We examined the effects of pre-analysis acidification on two size fractions of

    sediment organic matter (SOM) from marine and freshwater wetlands and pure muscle tissue of a

    common freshwater invertebrate (Cherax destructor). The elemental content and isotopic ratios of

    carbon and nitrogen were compared between paired samples of acidified and control treatments. Our

    results showed that acidification does not affect the elemental or isotopic values of freshwater SOM.

    In the marine environment acidification depleted the d13C and d15N values of the fine fraction of

    saltmarsh and d15N values of mangrove fine SOM. Whilst acidification did not change the elemental

    content of invertebrate muscle tissue, the d13C and d15N values were affected. We recommend to

    researchers considering using acidification techniques on material prepared for stable isotope

    analysis that a formal assessment of the effect of acidification on their particular sample type should

    be undertaken. Further detailed investigation to understand the impact of acidification on elemental

    and isotopic values of organic matter and muscular tissues is required.

UOW Authors


  •   Mazumder, Debashish (external author)
  •   Iles, Jordan (external author)
  •   Kelleway, Jeffrey
  •   Kobayashi, Tsuyoshi (external author)
  •   Knowles, Lisa (external author)
  •   Saintilan, Neil (external author)
  •   Hollins, Suzanne E. (external author)

Publication Date


  • 2010

Citation


  • Mazumder, D., Iles, J., Kelleway, J., Kobayashi, T., Knowles, L., Saintilan, N. & Hollins, S. (2010). Effect of acidification on elemental and isotopic compositions of sediment organic matter and macro‐invertebrate muscle tissues in food web research. Rapid Communications in Mass Spectrometry, 24 2938-2942.

Scopus Eid


  • 2-s2.0-77956943196

Number Of Pages


  • 4

Start Page


  • 2938

End Page


  • 2942

Volume


  • 24

Place Of Publication


  • United Kingdom