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Crystallization and preliminary X-ray crystallographic studies of Escherichia coli xanthine phosphoribosyltransferase

Journal Article


Abstract


  • Xanthine phosphoribosyltransferase (XPRT; EC 2.4.2.22) from Escherichia coli is a purine salvage enzyme which synthesizes the nucleotides GMP, XMP, and IMP. A mutant C59A, which is more stable than wild-type XPRT while retaining high activity, has been prepared and crystallized to give three different crystal forms (A, B, and C). Form A crystals are orthorhombic (P21212), with unit cell dimensions a = 59.2 Å, b = 92.9 Å, c = 53.2 Å. Form B crystals are monoclinic (C2) with unit cell dimensions a = 84.4 Å, b = 70.8 Å, c = 54.1 Å, and β = 113.4° and form C crystals are tetragonal (P41212 or P43212) with unit cell dimensions a,b = 94 Å, c = 167.6 Å. Wild-type XPRT and a selenomethionine derivative of C59A XPRT have also been crystallized in the orthorhombic form. The selenomethionine derivative was prepared by expressing XPRT in the usual E. coli strain without the need for a methionine auxotroph. Cells were grown in a methionine-deficient medium supplemented with selenomethionine which gave > 95% incorporation. Both the wild-type and selenomethionine C59A XPRT crystals are isomorphous with C59A form A crystals.

UOW Authors


  •   Vos, Siska (external author)
  •   de Jersey, John (external author)
  •   Martin, Jennifer

Publication Date


  • 1996

Citation


  • Vos, S., de Jersey, J. & Martin, J. L. (1996). Crystallization and preliminary X-ray crystallographic studies of Escherichia coli xanthine phosphoribosyltransferase. Journal of Structural Biology, 116 (2), 330-334.

Scopus Eid


  • 2-s2.0-0029931489

Number Of Pages


  • 4

Start Page


  • 330

End Page


  • 334

Volume


  • 116

Issue


  • 2

Place Of Publication


  • United States

Abstract


  • Xanthine phosphoribosyltransferase (XPRT; EC 2.4.2.22) from Escherichia coli is a purine salvage enzyme which synthesizes the nucleotides GMP, XMP, and IMP. A mutant C59A, which is more stable than wild-type XPRT while retaining high activity, has been prepared and crystallized to give three different crystal forms (A, B, and C). Form A crystals are orthorhombic (P21212), with unit cell dimensions a = 59.2 Å, b = 92.9 Å, c = 53.2 Å. Form B crystals are monoclinic (C2) with unit cell dimensions a = 84.4 Å, b = 70.8 Å, c = 54.1 Å, and β = 113.4° and form C crystals are tetragonal (P41212 or P43212) with unit cell dimensions a,b = 94 Å, c = 167.6 Å. Wild-type XPRT and a selenomethionine derivative of C59A XPRT have also been crystallized in the orthorhombic form. The selenomethionine derivative was prepared by expressing XPRT in the usual E. coli strain without the need for a methionine auxotroph. Cells were grown in a methionine-deficient medium supplemented with selenomethionine which gave > 95% incorporation. Both the wild-type and selenomethionine C59A XPRT crystals are isomorphous with C59A form A crystals.

UOW Authors


  •   Vos, Siska (external author)
  •   de Jersey, John (external author)
  •   Martin, Jennifer

Publication Date


  • 1996

Citation


  • Vos, S., de Jersey, J. & Martin, J. L. (1996). Crystallization and preliminary X-ray crystallographic studies of Escherichia coli xanthine phosphoribosyltransferase. Journal of Structural Biology, 116 (2), 330-334.

Scopus Eid


  • 2-s2.0-0029931489

Number Of Pages


  • 4

Start Page


  • 330

End Page


  • 334

Volume


  • 116

Issue


  • 2

Place Of Publication


  • United States