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Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells

Journal Article


Abstract


  • There are a vast range of diseases and disorders that are neurocristopathic in origin, including Hirschsprung's disease, pheochromocytoma, familial dysautonomia, craniofacial disorders, and melanomas. Having a source of human neural crest cells is highly valuable for investigating potential treatments for such diseases. This chapter describes a robust and well-characterized protocol for deriving neural crest from human pluripotent stem cells (hPSCs), which can then be differentiated to neuronal and non-neuronal lineages. The protocol is adapted to suit hPSC maintenance as a monolayer bulk culture or as manual-passaged colonies, which makes it widely applicable to researchers that may use different systems for hPSC maintenance.

Authors


  •   Abu-Bonsrah, Kwaku Dad (external author)
  •   Viventi, Serena (external author)
  •   Newgreen, Donald F. (external author)
  •   Dottori, Mirella

Publication Date


  • 2019

Citation


  • Abu-Bonsrah, K., Viventi, S., Newgreen, D. F. & Dottori, M. (2019). Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells. Methods in Molecular Biology, 1976 37-47.

Scopus Eid


  • 2-s2.0-85064807210

Number Of Pages


  • 10

Start Page


  • 37

End Page


  • 47

Volume


  • 1976

Place Of Publication


  • United States

Abstract


  • There are a vast range of diseases and disorders that are neurocristopathic in origin, including Hirschsprung's disease, pheochromocytoma, familial dysautonomia, craniofacial disorders, and melanomas. Having a source of human neural crest cells is highly valuable for investigating potential treatments for such diseases. This chapter describes a robust and well-characterized protocol for deriving neural crest from human pluripotent stem cells (hPSCs), which can then be differentiated to neuronal and non-neuronal lineages. The protocol is adapted to suit hPSC maintenance as a monolayer bulk culture or as manual-passaged colonies, which makes it widely applicable to researchers that may use different systems for hPSC maintenance.

Authors


  •   Abu-Bonsrah, Kwaku Dad (external author)
  •   Viventi, Serena (external author)
  •   Newgreen, Donald F. (external author)
  •   Dottori, Mirella

Publication Date


  • 2019

Citation


  • Abu-Bonsrah, K., Viventi, S., Newgreen, D. F. & Dottori, M. (2019). Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells. Methods in Molecular Biology, 1976 37-47.

Scopus Eid


  • 2-s2.0-85064807210

Number Of Pages


  • 10

Start Page


  • 37

End Page


  • 47

Volume


  • 1976

Place Of Publication


  • United States