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RecFOR epistasis group: RecF and RecO have distinct localizations and functions in Escherichia coli

Journal Article


Abstract


  • In bacteria, genetic recombination is a major mechanism for DNA repair. The RecF, RecO and RecR proteins are proposed to initiate recombination by loading the RecA recombinase onto DNA. However, the biophysical mechanisms underlying this process remain poorly understood. Here, we used genetics and single-molecule fluorescence microscopy to investigate whether RecF and RecO function together, or separately, in live Escherichia coli cells. We identified conditions in which RecF and RecO functions are genetically separable. Single-molecule imaging revealed key differences in the spatiotemporal behaviours of RecF and RecO. RecF foci frequently colocalize with replisome markers. In response to DNA damage, colocalization increases and RecF dimerizes. The majority of RecF foci are dependent on RecR. Conversely, RecO foci occur infrequently, rarely colocalize with replisomes or RecF and are largely independent of RecR. In response to DNA damage, RecO foci appeared to spatially redistribute, occupying a region close to the cell membrane. These observations indicate that RecF and RecO have distinct functions in the DNA damage response. The observed localization of RecF to the replisome supports the notion that RecF helps to maintain active DNA replication in cells carrying DNA damage.

Authors


  •   Henrikus, Sarah (external author)
  •   Henry, Camille (external author)
  •   Ghodke, Harshad
  •   Wood, Elizabeth A. (external author)
  •   Mbele, Neema (external author)
  •   Saxena, Roopashi (external author)
  •   Basu, Upasana (external author)
  •   van Oijen, Antoine M.
  •   Cox, Michael M. (external author)
  •   Robinson, Andrew

Publication Date


  • 2019

Citation


  • Henrikus, S. S., Henry, C., Ghodke, H., Wood, E. A., Mbele, N., Saxena, R., Basu, U., van Oijen, A. M., Cox, M. M. & Robinson, A. (2019). RecFOR epistasis group: RecF and RecO have distinct localizations and functions in Escherichia coli. Nucleic Acids Research, 47 (6), 2946-2965.

Scopus Eid


  • 2-s2.0-85064526715

Number Of Pages


  • 19

Start Page


  • 2946

End Page


  • 2965

Volume


  • 47

Issue


  • 6

Place Of Publication


  • United Kingdom

Abstract


  • In bacteria, genetic recombination is a major mechanism for DNA repair. The RecF, RecO and RecR proteins are proposed to initiate recombination by loading the RecA recombinase onto DNA. However, the biophysical mechanisms underlying this process remain poorly understood. Here, we used genetics and single-molecule fluorescence microscopy to investigate whether RecF and RecO function together, or separately, in live Escherichia coli cells. We identified conditions in which RecF and RecO functions are genetically separable. Single-molecule imaging revealed key differences in the spatiotemporal behaviours of RecF and RecO. RecF foci frequently colocalize with replisome markers. In response to DNA damage, colocalization increases and RecF dimerizes. The majority of RecF foci are dependent on RecR. Conversely, RecO foci occur infrequently, rarely colocalize with replisomes or RecF and are largely independent of RecR. In response to DNA damage, RecO foci appeared to spatially redistribute, occupying a region close to the cell membrane. These observations indicate that RecF and RecO have distinct functions in the DNA damage response. The observed localization of RecF to the replisome supports the notion that RecF helps to maintain active DNA replication in cells carrying DNA damage.

Authors


  •   Henrikus, Sarah (external author)
  •   Henry, Camille (external author)
  •   Ghodke, Harshad
  •   Wood, Elizabeth A. (external author)
  •   Mbele, Neema (external author)
  •   Saxena, Roopashi (external author)
  •   Basu, Upasana (external author)
  •   van Oijen, Antoine M.
  •   Cox, Michael M. (external author)
  •   Robinson, Andrew

Publication Date


  • 2019

Citation


  • Henrikus, S. S., Henry, C., Ghodke, H., Wood, E. A., Mbele, N., Saxena, R., Basu, U., van Oijen, A. M., Cox, M. M. & Robinson, A. (2019). RecFOR epistasis group: RecF and RecO have distinct localizations and functions in Escherichia coli. Nucleic Acids Research, 47 (6), 2946-2965.

Scopus Eid


  • 2-s2.0-85064526715

Number Of Pages


  • 19

Start Page


  • 2946

End Page


  • 2965

Volume


  • 47

Issue


  • 6

Place Of Publication


  • United Kingdom