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Isotope labeling reveals contribution of newly fixed carbon to carbon storage and monoterpenes production under water deficit and carbon limitation

Journal Article


Abstract


  • Secondary metabolites play important roles in plant responses to environmental stress but may also represent a large carbon (C) cost, resulting in trade-offs with other C sinks like growth and storage. However, it remains uncertain how such trade-offs may vary with changes in resource availability including water and CO 2 availability. We conducted a glasshouse experiment with peppermint (Mentha x piperita L.) exposed to four treatments: control (sufficient irrigation and near-ambient CO 2 ), water deficit (50% irrigation and near-ambient CO 2 ), CO 2 limitation (sufficient irrigation and below-ambient CO 2 ) and the combination of water and CO 2 deficits. Continuous 13 CO 2 labelling was used to trace allocation of newly-assimilated C. Concentrations of soluble sugars significantly increased under water deficit but decreased along with aboveground biomass under low CO 2 , while monoterpene concentrations remained relatively constant, independent of treatments. Under water deficit, there were no differences in allocation of new vs old C to monoterpenes production, structural growth and storage; plants grown under low CO 2 even invested proportionally more newly-assimilated C for monoterpenes production, suggesting a preferential allocation to defense at the expense of growth. We concluded that C allocation to monoterpenes is actively regulated in coordination with growth and storage under water and C stresses, consistent with an optimal defense strategy to protect young tissues.

UOW Authors


  •   Huang, Jianbei (external author)
  •   Forkelova, Lenka (external author)
  •   Unsicker, Sybille (external author)
  •   Forkel, Matthias (external author)
  •   Griffith, David
  •   Trumbore, Susan (external author)
  •   Hartmann, Henrik (external author)

Publication Date


  • 2019

Citation


  • Huang, J., Forkelova, L., Unsicker, S. B., Forkel, M., Griffith, D. W.T., Trumbore, S. & Hartmann, H. (2019). Isotope labeling reveals contribution of newly fixed carbon to carbon storage and monoterpenes production under water deficit and carbon limitation. Environmental and Experimental Botany, 162 333-344.

Scopus Eid


  • 2-s2.0-85063065358

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers1/635

Number Of Pages


  • 11

Start Page


  • 333

End Page


  • 344

Volume


  • 162

Place Of Publication


  • Netherlands

Abstract


  • Secondary metabolites play important roles in plant responses to environmental stress but may also represent a large carbon (C) cost, resulting in trade-offs with other C sinks like growth and storage. However, it remains uncertain how such trade-offs may vary with changes in resource availability including water and CO 2 availability. We conducted a glasshouse experiment with peppermint (Mentha x piperita L.) exposed to four treatments: control (sufficient irrigation and near-ambient CO 2 ), water deficit (50% irrigation and near-ambient CO 2 ), CO 2 limitation (sufficient irrigation and below-ambient CO 2 ) and the combination of water and CO 2 deficits. Continuous 13 CO 2 labelling was used to trace allocation of newly-assimilated C. Concentrations of soluble sugars significantly increased under water deficit but decreased along with aboveground biomass under low CO 2 , while monoterpene concentrations remained relatively constant, independent of treatments. Under water deficit, there were no differences in allocation of new vs old C to monoterpenes production, structural growth and storage; plants grown under low CO 2 even invested proportionally more newly-assimilated C for monoterpenes production, suggesting a preferential allocation to defense at the expense of growth. We concluded that C allocation to monoterpenes is actively regulated in coordination with growth and storage under water and C stresses, consistent with an optimal defense strategy to protect young tissues.

UOW Authors


  •   Huang, Jianbei (external author)
  •   Forkelova, Lenka (external author)
  •   Unsicker, Sybille (external author)
  •   Forkel, Matthias (external author)
  •   Griffith, David
  •   Trumbore, Susan (external author)
  •   Hartmann, Henrik (external author)

Publication Date


  • 2019

Citation


  • Huang, J., Forkelova, L., Unsicker, S. B., Forkel, M., Griffith, D. W.T., Trumbore, S. & Hartmann, H. (2019). Isotope labeling reveals contribution of newly fixed carbon to carbon storage and monoterpenes production under water deficit and carbon limitation. Environmental and Experimental Botany, 162 333-344.

Scopus Eid


  • 2-s2.0-85063065358

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers1/635

Number Of Pages


  • 11

Start Page


  • 333

End Page


  • 344

Volume


  • 162

Place Of Publication


  • Netherlands