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Enhanced Peroxidase Mimetic Activity of Porous Iron Oxide Nanoflakes

Journal Article


Abstract


  • Porous nanomaterials with superior peroxidase mimetic activity (nanozyme) at room temperature have gained increasing attention as potential alternatives to natural peroxidase enzymes. Herein, we report the application of porous iron oxide nanoflakes (IONFs), synthesized using the combination of solvothermal method and high-temperature calcination as peroxidase nanozyme for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. The four IONF catalysts possess porous structures with a wide pore size distribution between 2–30 nm and high specific surface areas around to 200 m2 g−1. The increase of calcination temperature of the IONFs from 250 °C to 400 °C resulted in a gradual decrease in their specific surface area and Michaelis-Menten constant (Km) for TMB oxidation. The optimum IONF sample showed a much lower Km at 0.24 mM (towards TMB) compared to natural enzyme horseradish peroxidase (HRP) at 0.434 mM, revealing the promising potential of the as-prepared IONFs as alternatives to HRP for biosensing applications.

UOW Authors


  •   Tanaka, Shunsuke (external author)
  •   Masud, Mostafa (external author)
  •   Kaneti, Yusuf (external author)
  •   Shiddiky, Muhammad J. A. (external author)
  •   Fatehmulla, Amanullah (external author)
  •   Aldhafiri, Abdullah (external author)
  •   Farooq, W Aslam. (external author)
  •   Bando, Yoshio
  •   Hossain, Md Shahriar
  •   Yamauchi, Yusuke (external author)

Publication Date


  • 2019

Citation


  • Tanaka, S., Masud, M. Kamal., Kaneti, Y. Valentino., Shiddiky, M. J. A., Fatehmulla, A., Aldhafiri, A. M., Farooq, W. Aslam., Bando, Y., Hossain, M. A. & Yamauchi, Y. (2019). Enhanced Peroxidase Mimetic Activity of Porous Iron Oxide Nanoflakes. ChemNanoMat: chemistry of nanomaterials for energy, biology and more, 5 506-513.

Scopus Eid


  • 2-s2.0-85061101900

Number Of Pages


  • 7

Start Page


  • 506

End Page


  • 513

Volume


  • 5

Place Of Publication


  • Germany

Abstract


  • Porous nanomaterials with superior peroxidase mimetic activity (nanozyme) at room temperature have gained increasing attention as potential alternatives to natural peroxidase enzymes. Herein, we report the application of porous iron oxide nanoflakes (IONFs), synthesized using the combination of solvothermal method and high-temperature calcination as peroxidase nanozyme for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. The four IONF catalysts possess porous structures with a wide pore size distribution between 2–30 nm and high specific surface areas around to 200 m2 g−1. The increase of calcination temperature of the IONFs from 250 °C to 400 °C resulted in a gradual decrease in their specific surface area and Michaelis-Menten constant (Km) for TMB oxidation. The optimum IONF sample showed a much lower Km at 0.24 mM (towards TMB) compared to natural enzyme horseradish peroxidase (HRP) at 0.434 mM, revealing the promising potential of the as-prepared IONFs as alternatives to HRP for biosensing applications.

UOW Authors


  •   Tanaka, Shunsuke (external author)
  •   Masud, Mostafa (external author)
  •   Kaneti, Yusuf (external author)
  •   Shiddiky, Muhammad J. A. (external author)
  •   Fatehmulla, Amanullah (external author)
  •   Aldhafiri, Abdullah (external author)
  •   Farooq, W Aslam. (external author)
  •   Bando, Yoshio
  •   Hossain, Md Shahriar
  •   Yamauchi, Yusuke (external author)

Publication Date


  • 2019

Citation


  • Tanaka, S., Masud, M. Kamal., Kaneti, Y. Valentino., Shiddiky, M. J. A., Fatehmulla, A., Aldhafiri, A. M., Farooq, W. Aslam., Bando, Y., Hossain, M. A. & Yamauchi, Y. (2019). Enhanced Peroxidase Mimetic Activity of Porous Iron Oxide Nanoflakes. ChemNanoMat: chemistry of nanomaterials for energy, biology and more, 5 506-513.

Scopus Eid


  • 2-s2.0-85061101900

Number Of Pages


  • 7

Start Page


  • 506

End Page


  • 513

Volume


  • 5

Place Of Publication


  • Germany