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A simple technique for development of fibres with programmable microsphere concentration gradients for local protein delivery

Journal Article


Abstract


  • Alginate has been a biologically viable option for controlled local delivery of bioactive molecules in vitro and in vivo. Specific bioactive molecule release profiles are achieved often by controlling polymer composition/concentration, which also determines the modulus of hydrogels. This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes. Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres. Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized. The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre). The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone. In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres. The “programmable” microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.

Publication Date


  • 2019

Citation


  • Mehrpouya, F., Yue, Z., Romeo, T., Gorkin, R., Kapsa, R. M. I., Moulton, S. E. & Wallace, G. G. (2019). A simple technique for development of fibres with programmable microsphere concentration gradients for local protein delivery. Journal of Materials Chemistry B, 7 (4), 556-565.

Scopus Eid


  • 2-s2.0-85060395816

Number Of Pages


  • 9

Start Page


  • 556

End Page


  • 565

Volume


  • 7

Issue


  • 4

Place Of Publication


  • United Kingdom

Abstract


  • Alginate has been a biologically viable option for controlled local delivery of bioactive molecules in vitro and in vivo. Specific bioactive molecule release profiles are achieved often by controlling polymer composition/concentration, which also determines the modulus of hydrogels. This largely limits alginate-mediated bioactive molecule delivery to single-factors of uniform concentration applications, rather than applications that may require (multiple) bioactive molecules delivered at a concentration gradient for chemotactic purposes. Here we report a two-phase PLGA/alginate delivery system composed of protein-laden poly-d,l-lactic-co-glycolic acid (PLGA) microspheres wet-spun into alginate fibres. Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) was used as a model protein and the developed structures were characterized. The fabrication system devised was shown to produce wet-spun fibres with a protein concentration gradient (G-Alg/PLGA fibre). The two-phase delivery matrices display retarded FITC-BSA release in both initial and late stages compared to release from the PLGA microspheres or alginate fibre alone. In addition, incorporation of higher concentrations of protein-loaded PLGA microspheres increased protein release compared to the fibres with lower concentrations of BSA-loaded microspheres. The “programmable” microsphere concentration gradient fibre methodology presented here may enable development of novel alginate scaffolds with the ability to guide tissue regeneration through tightly-controlled release of one or more proteins in highly defined spatio-temporal configurations.

Publication Date


  • 2019

Citation


  • Mehrpouya, F., Yue, Z., Romeo, T., Gorkin, R., Kapsa, R. M. I., Moulton, S. E. & Wallace, G. G. (2019). A simple technique for development of fibres with programmable microsphere concentration gradients for local protein delivery. Journal of Materials Chemistry B, 7 (4), 556-565.

Scopus Eid


  • 2-s2.0-85060395816

Number Of Pages


  • 9

Start Page


  • 556

End Page


  • 565

Volume


  • 7

Issue


  • 4

Place Of Publication


  • United Kingdom