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Shining a spotlight on DNA: Single-molecule methods to visualise DNA

Journal Article


Abstract


  • The ability to watch single molecules of DNA has revolutionised how we study biological transactions concerning nucleic acids. Many strategies have been developed to manipulate DNA molecules to investigate mechanical properties, dynamics and protein–DNA interactions. Imaging methods using small molecules and protein-based probes to visualise DNA have propelled our understanding of complex biochemical reactions involving DNA. This review focuses on summarising some of the methodological developments made to visualise individual DNA molecules and discusses how these probes have been used in single-molecule biophysical assays.

Authors


Publication Date


  • 2019

Citation


  • Kaur, G., Lewis, J. S. & van Oijen, A. M. (2019). Shining a spotlight on DNA: Single-molecule methods to visualise DNA. Molecules, 24 (3), 491-1-491-20.

Scopus Eid


  • 2-s2.0-85060680069

Start Page


  • 491-1

End Page


  • 491-20

Volume


  • 24

Issue


  • 3

Place Of Publication


  • Switzerland

Abstract


  • The ability to watch single molecules of DNA has revolutionised how we study biological transactions concerning nucleic acids. Many strategies have been developed to manipulate DNA molecules to investigate mechanical properties, dynamics and protein–DNA interactions. Imaging methods using small molecules and protein-based probes to visualise DNA have propelled our understanding of complex biochemical reactions involving DNA. This review focuses on summarising some of the methodological developments made to visualise individual DNA molecules and discusses how these probes have been used in single-molecule biophysical assays.

Authors


Publication Date


  • 2019

Citation


  • Kaur, G., Lewis, J. S. & van Oijen, A. M. (2019). Shining a spotlight on DNA: Single-molecule methods to visualise DNA. Molecules, 24 (3), 491-1-491-20.

Scopus Eid


  • 2-s2.0-85060680069

Start Page


  • 491-1

End Page


  • 491-20

Volume


  • 24

Issue


  • 3

Place Of Publication


  • Switzerland