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Aripiprazole and haloperidol protect neurite lesions via reducing excessive D2R-DISC1 complex formation

Journal Article


Abstract


  • Dopamine D2 receptor (D2R) hyperactivity causes altered brain development and later produces onset of symptoms mimicking schizophrenia. It is known that D2R interacts with disrupted in schizophrenia 1 (DISC1); however, the effect of D2R-DISC1 interaction in intracellular signalling and neurite growth has not been studied. This study investigated the effect of D2R over-activation on Akt-GSK3β signalling and neurite morphology in cortical neurons. Over-activation of D2Rs caused neurite lesions, which were associated with decreased protein kinase B (Akt) and glycogen synthase kinase 3 beta (GSK3β) phosphorylation in cortical neurons. The antipsychotic drug aripiprazole was more effective in the prevention of neurite lesions than haloperidol. Unlike haloperidol, aripiprazole prevented downregulation of phospho (p) Akt-pGSK3β induced by D2R hyperactivity, indicating involvement of different pathways. D2Rs were hyperactive in cortical neurons of mice with DISC1 mutation, which caused more severe neurite lesions in cortical neurons treated with quinpirole. Immunofluorescent staining for Ca2+/calmodulin-dependent protein kinase II (CaMKII) confirmed that cortical pyramidal neurons were involved in the D2R hyperactivity-induced neurite lesions. Using the fluorescence resonance energy transfer (FRET) technique, we provide direct evidence that D2R hyperactivity led to D2R-DISC1 complex formation, which altered pGSK3β signalling. This study showed that D2R hyperactivity-induced D2R-DISC1 complex formation is associated with decreased pAkt-pGSK3β signalling and in turn, caused neurite impairment. Aripiprazole and haloperidol prevented the impairment of neurite growth but appeared to do so via different intracellular signalling pathways.

Authors


  •   Zheng, Peng (external author)
  •   Hu, Minmin (external author)
  •   Xie, Yuanyi (external author)
  •   Yu, Yinghua
  •   Jaaro-Peled, Hanna (external author)
  •   Huang, Xu-Feng

Publication Date


  • 2019

Citation


  • Zheng, P., Hu, M., Xie, Y., Yu, Y., Jaaro-Peled, H. & Huang, X. (2019). Aripiprazole and haloperidol protect neurite lesions via reducing excessive D2R-DISC1 complex formation. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 92 59-69.

Scopus Eid


  • 2-s2.0-85059351783

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1336

Number Of Pages


  • 10

Start Page


  • 59

End Page


  • 69

Volume


  • 92

Place Of Publication


  • United States

Abstract


  • Dopamine D2 receptor (D2R) hyperactivity causes altered brain development and later produces onset of symptoms mimicking schizophrenia. It is known that D2R interacts with disrupted in schizophrenia 1 (DISC1); however, the effect of D2R-DISC1 interaction in intracellular signalling and neurite growth has not been studied. This study investigated the effect of D2R over-activation on Akt-GSK3β signalling and neurite morphology in cortical neurons. Over-activation of D2Rs caused neurite lesions, which were associated with decreased protein kinase B (Akt) and glycogen synthase kinase 3 beta (GSK3β) phosphorylation in cortical neurons. The antipsychotic drug aripiprazole was more effective in the prevention of neurite lesions than haloperidol. Unlike haloperidol, aripiprazole prevented downregulation of phospho (p) Akt-pGSK3β induced by D2R hyperactivity, indicating involvement of different pathways. D2Rs were hyperactive in cortical neurons of mice with DISC1 mutation, which caused more severe neurite lesions in cortical neurons treated with quinpirole. Immunofluorescent staining for Ca2+/calmodulin-dependent protein kinase II (CaMKII) confirmed that cortical pyramidal neurons were involved in the D2R hyperactivity-induced neurite lesions. Using the fluorescence resonance energy transfer (FRET) technique, we provide direct evidence that D2R hyperactivity led to D2R-DISC1 complex formation, which altered pGSK3β signalling. This study showed that D2R hyperactivity-induced D2R-DISC1 complex formation is associated with decreased pAkt-pGSK3β signalling and in turn, caused neurite impairment. Aripiprazole and haloperidol prevented the impairment of neurite growth but appeared to do so via different intracellular signalling pathways.

Authors


  •   Zheng, Peng (external author)
  •   Hu, Minmin (external author)
  •   Xie, Yuanyi (external author)
  •   Yu, Yinghua
  •   Jaaro-Peled, Hanna (external author)
  •   Huang, Xu-Feng

Publication Date


  • 2019

Citation


  • Zheng, P., Hu, M., Xie, Y., Yu, Y., Jaaro-Peled, H. & Huang, X. (2019). Aripiprazole and haloperidol protect neurite lesions via reducing excessive D2R-DISC1 complex formation. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 92 59-69.

Scopus Eid


  • 2-s2.0-85059351783

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1336

Number Of Pages


  • 10

Start Page


  • 59

End Page


  • 69

Volume


  • 92

Place Of Publication


  • United States