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In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy

Journal Article


Abstract


  • Vascular immune-inflammatory responses play a crucial role in the progression and outcome of

    atherosclerosis. The ability to assess localized inflammation through detection of specific vascular

    inflammatory biomarkers would significantly improve cardiovascular risk assessment and

    management; however, no multi-parameter molecular imaging technologies have been established

    to date. Here, we report the targeted in vivo imaging of multiple vascular biomarkers using

    antibody-functionalized nanoparticles and surface-enhanced Raman scattering (SERS).

    Methods: A series of antibody-functionalized gold nanoprobes (BFNP) were designed containing

    unique Raman signals in order to detect intercellular adhesion molecule 1 (ICAM-1), vascular cell

    adhesion molecule 1 (VCAM-1) and P-selectin using SERS.

    Results: SERS and BFNP were utilized to detect, discriminate and quantify ICAM-1, VCAM-1 and

    P-selectin in vitro on human endothelial cells and ex vivo in human coronary arteries. Ultimately,

    non-invasive multiplex imaging of adhesion molecules in a humanized mouse model was

    demonstrated in vivo following intravenous injection of the nanoprobes.

    Conclusion: This study demonstrates that multiplexed SERS-based molecular imaging can indicate

    the status of vascular inflammation in vivo and gives promise for SERS as a clinical imaging technique

    for cardiovascular disease in the future.

UOW Authors


  •   Noonan, Jonathan (external author)
  •   Asiala, Steven M. (external author)
  •   Grassia, Gianluca (external author)
  •   MacRitchie, Neil (external author)
  •   Gracie, Kirsten (external author)
  •   Carson, Jake (external author)
  •   Moores, Matt T.
  •   Girolami, Mark (external author)
  •   Bradshaw, Angela C. (external author)
  •   Guzik, Tomasz J. (external author)
  •   Meehan, Gavin R. (external author)
  •   Scales, Hannah E. (external author)
  •   Brewer, James M. (external author)
  •   McInnes, Iain B. (external author)
  •   Sattar, Naveed (external author)
  •   Faulds, Karen (external author)
  •   Garside, Paul (external author)
  •   Graham, Duncan (external author)
  •   Maffia, Pasquale (external author)

Publication Date


  • 2018

Citation


  • Noonan, J., Asiala, S. M., Grassia, G., MacRitchie, N., Gracie, K., Carson, J., Moores, M., Girolami, M., Bradshaw, A. C., Guzik, T. J., Meehan, G. R., Scales, H. E., Brewer, J. M., McInnes, I. B., Sattar, N., Faulds, K., Garside, P., Graham, D. & Maffia, P. (2018). In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy. Theranostics, 8 (22), 6195-6209.

Scopus Eid


  • 2-s2.0-85059321280

Number Of Pages


  • 14

Start Page


  • 6195

End Page


  • 6209

Volume


  • 8

Issue


  • 22

Place Of Publication


  • Australia

Abstract


  • Vascular immune-inflammatory responses play a crucial role in the progression and outcome of

    atherosclerosis. The ability to assess localized inflammation through detection of specific vascular

    inflammatory biomarkers would significantly improve cardiovascular risk assessment and

    management; however, no multi-parameter molecular imaging technologies have been established

    to date. Here, we report the targeted in vivo imaging of multiple vascular biomarkers using

    antibody-functionalized nanoparticles and surface-enhanced Raman scattering (SERS).

    Methods: A series of antibody-functionalized gold nanoprobes (BFNP) were designed containing

    unique Raman signals in order to detect intercellular adhesion molecule 1 (ICAM-1), vascular cell

    adhesion molecule 1 (VCAM-1) and P-selectin using SERS.

    Results: SERS and BFNP were utilized to detect, discriminate and quantify ICAM-1, VCAM-1 and

    P-selectin in vitro on human endothelial cells and ex vivo in human coronary arteries. Ultimately,

    non-invasive multiplex imaging of adhesion molecules in a humanized mouse model was

    demonstrated in vivo following intravenous injection of the nanoprobes.

    Conclusion: This study demonstrates that multiplexed SERS-based molecular imaging can indicate

    the status of vascular inflammation in vivo and gives promise for SERS as a clinical imaging technique

    for cardiovascular disease in the future.

UOW Authors


  •   Noonan, Jonathan (external author)
  •   Asiala, Steven M. (external author)
  •   Grassia, Gianluca (external author)
  •   MacRitchie, Neil (external author)
  •   Gracie, Kirsten (external author)
  •   Carson, Jake (external author)
  •   Moores, Matt T.
  •   Girolami, Mark (external author)
  •   Bradshaw, Angela C. (external author)
  •   Guzik, Tomasz J. (external author)
  •   Meehan, Gavin R. (external author)
  •   Scales, Hannah E. (external author)
  •   Brewer, James M. (external author)
  •   McInnes, Iain B. (external author)
  •   Sattar, Naveed (external author)
  •   Faulds, Karen (external author)
  •   Garside, Paul (external author)
  •   Graham, Duncan (external author)
  •   Maffia, Pasquale (external author)

Publication Date


  • 2018

Citation


  • Noonan, J., Asiala, S. M., Grassia, G., MacRitchie, N., Gracie, K., Carson, J., Moores, M., Girolami, M., Bradshaw, A. C., Guzik, T. J., Meehan, G. R., Scales, H. E., Brewer, J. M., McInnes, I. B., Sattar, N., Faulds, K., Garside, P., Graham, D. & Maffia, P. (2018). In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy. Theranostics, 8 (22), 6195-6209.

Scopus Eid


  • 2-s2.0-85059321280

Number Of Pages


  • 14

Start Page


  • 6195

End Page


  • 6209

Volume


  • 8

Issue


  • 22

Place Of Publication


  • Australia