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Viral-free generation and characterization of a human induced pluripotent stem cell line from dermal fibroblasts

Journal Article


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Abstract


  • Peripheral dermal fibroblasts (DF) from a healthy 56 year old female were obtained from the Centre for Healthy Brain Ageing (CHeBA) Biobank, University of New South Wales, under the material transfer agreement with the University of Wollongong. DFs were reprogrammed via mRNA-delivered transcription factors into induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed to be pluripotent, capable of three germ layer differentiation and are thus a useful resource for creating iPSC-derived healthy human cells of any lineage.

Publication Date


  • 2018

Citation


  • Engel, M., Balez, R., Sanz Munoz, S., Cabral-Da-Silva, M. Castro., Stevens, C. Helen., Bax, M., Do-Ha, D., Sidhu, K., Sachdev, P. & Ooi, L. (2018). Viral-free generation and characterization of a human induced pluripotent stem cell line from dermal fibroblasts. Stem Cell Research, 32 135-138.

Scopus Eid


  • 2-s2.0-85054067908

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=2316&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1289

Number Of Pages


  • 3

Start Page


  • 135

End Page


  • 138

Volume


  • 32

Place Of Publication


  • Netherlands

Abstract


  • Peripheral dermal fibroblasts (DF) from a healthy 56 year old female were obtained from the Centre for Healthy Brain Ageing (CHeBA) Biobank, University of New South Wales, under the material transfer agreement with the University of Wollongong. DFs were reprogrammed via mRNA-delivered transcription factors into induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed to be pluripotent, capable of three germ layer differentiation and are thus a useful resource for creating iPSC-derived healthy human cells of any lineage.

Publication Date


  • 2018

Citation


  • Engel, M., Balez, R., Sanz Munoz, S., Cabral-Da-Silva, M. Castro., Stevens, C. Helen., Bax, M., Do-Ha, D., Sidhu, K., Sachdev, P. & Ooi, L. (2018). Viral-free generation and characterization of a human induced pluripotent stem cell line from dermal fibroblasts. Stem Cell Research, 32 135-138.

Scopus Eid


  • 2-s2.0-85054067908

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=2316&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1289

Number Of Pages


  • 3

Start Page


  • 135

End Page


  • 138

Volume


  • 32

Place Of Publication


  • Netherlands