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Increasing Acyl CoA thioesterase activity alters phospholipid profle without effect on insulin action in skeletal muscle of rats

Journal Article


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Abstract


  • Increased lipid metabolism in muscle is associated with insulin resistance and therefore, many

    strategies have been employed to alter fatty acid metabolism and study the impact on insulin

    action. Metabolism of fatty acid requires activation to fatty acyl CoA by Acyl CoA synthases (ACSL)

    and fatty acyl CoA can be hydrolysed by Acyl CoA thioesterases (Acot). Thioesterase activity is

    low in muscle, so we overexpressed Acot7 in muscle of chow and high-fat diet (HFD) rats and

    investigated effects on insulin action. Acot7 overexpression modified specific phosphatidylcholine and

    phosphatidylethanolamine species in tibialis muscle of chow rats to levels similar to those observed

    in control HFD muscle. The changes in phospholipid species did not alter glucose uptake in tibialis

    muscle under hyperinsulinaemic/euglycaemic clamped conditions. Acot7 overexpression in white

    extensor digitorum longus (EDL) muscle increased complete fatty acid oxidation ex-vivo but was not

    associated with any changes in glucose uptake in-vivo, however overexpression of Acot7 in red EDL

    reduced insulin-stimulated glucose uptake in-vivo which correlated with increased incomplete fatty acid

    oxidation ex-vivo. In summary, although overexpression of Acot7 in muscle altered some aspects of lipid

    profile and metabolism in muscle, this had no major effect on insulin-stimulated glucose uptake.

Authors


  •   Bakshi, Ishita (external author)
  •   Brown, Simon H. J. (external author)
  •   Brandon, Amanda (external author)
  •   Suryana, Eurwin (external author)
  •   Mitchell, Todd
  •   Turner, Nigel (external author)
  •   Cooney, Gregory J. (external author)

Publication Date


  • 2018

Citation


  • Bakshi, I., Brown, S. H. J., Brandon, A. E., Suryana, E., Mitchell, T. W., Turner, N. & Cooney, G. J. (2018). Increasing Acyl CoA thioesterase activity alters phospholipid profle without effect on insulin action in skeletal muscle of rats. Scientific Reports, 8 13967-1-13967-12.

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=1219&context=smhpapers1

Start Page


  • 13967-1

End Page


  • 13967-12

Volume


  • 8

Place Of Publication


  • United Kingdom

Abstract


  • Increased lipid metabolism in muscle is associated with insulin resistance and therefore, many

    strategies have been employed to alter fatty acid metabolism and study the impact on insulin

    action. Metabolism of fatty acid requires activation to fatty acyl CoA by Acyl CoA synthases (ACSL)

    and fatty acyl CoA can be hydrolysed by Acyl CoA thioesterases (Acot). Thioesterase activity is

    low in muscle, so we overexpressed Acot7 in muscle of chow and high-fat diet (HFD) rats and

    investigated effects on insulin action. Acot7 overexpression modified specific phosphatidylcholine and

    phosphatidylethanolamine species in tibialis muscle of chow rats to levels similar to those observed

    in control HFD muscle. The changes in phospholipid species did not alter glucose uptake in tibialis

    muscle under hyperinsulinaemic/euglycaemic clamped conditions. Acot7 overexpression in white

    extensor digitorum longus (EDL) muscle increased complete fatty acid oxidation ex-vivo but was not

    associated with any changes in glucose uptake in-vivo, however overexpression of Acot7 in red EDL

    reduced insulin-stimulated glucose uptake in-vivo which correlated with increased incomplete fatty acid

    oxidation ex-vivo. In summary, although overexpression of Acot7 in muscle altered some aspects of lipid

    profile and metabolism in muscle, this had no major effect on insulin-stimulated glucose uptake.

Authors


  •   Bakshi, Ishita (external author)
  •   Brown, Simon H. J. (external author)
  •   Brandon, Amanda (external author)
  •   Suryana, Eurwin (external author)
  •   Mitchell, Todd
  •   Turner, Nigel (external author)
  •   Cooney, Gregory J. (external author)

Publication Date


  • 2018

Citation


  • Bakshi, I., Brown, S. H. J., Brandon, A. E., Suryana, E., Mitchell, T. W., Turner, N. & Cooney, G. J. (2018). Increasing Acyl CoA thioesterase activity alters phospholipid profle without effect on insulin action in skeletal muscle of rats. Scientific Reports, 8 13967-1-13967-12.

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=1219&context=smhpapers1

Start Page


  • 13967-1

End Page


  • 13967-12

Volume


  • 8

Place Of Publication


  • United Kingdom