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Degradable 3D-Printed Hydrogels Based on Star-Shaped Copolypeptides

Journal Article


Abstract


  • We present a star copolypeptide-based hydrogel ink capable of structural microfabrication using 3D extrusion printing. The material comprises an amphiphilic block copolymer structure of poly(benzyl-l-glutamate)-b-oligo(l-valine), which spontaneously forms hydrogels through hydrophobic interactions. The chemical design allows the bulk phase of the hydrogel to remain intact after application of shear due to its self-recovery behavior. It is demonstrated that the composition of the materials is ideally suited for 3D printing with scaffolds capable of maintaining structural cohesion after extrusion. Post extrusion UV-triggered fixation of the printed structures is carried out, resulting in stable hydrogel constructs. The constructs were found to be degradable, exhibited favorable release of encapsulated molecular cargo, and do not appear to affect the metabolic health of the commonly used fibroblastic cell line Balb/3T3 in the absence of the reactive diluent N,N′-methylenebis(acrylamide). The star copolypeptide inks allow for rapid prototyping enabling the fabrication of defined intricate microstructures, providing a platform for complex scaffold development that would otherwise be unattainable with other processing techniques such as molding or casting.

Authors


  •   Murphy, Robert (external author)
  •   Walsh, David (external author)
  •   Hamilton, Charles (external author)
  •   Cryan, Sally-Ann (external author)
  •   in het Panhuis, Marc
  •   Heise, Andreas (external author)

Publication Date


  • 2018

Citation


  • Murphy, R., Walsh, D. P., Hamilton, C. A., Cryan, S., in het Panhuis, M. & Heise, A. (2018). Degradable 3D-Printed Hydrogels Based on Star-Shaped Copolypeptides. Biomacromolecules, 19 (7), 2691-2699.

Scopus Eid


  • 2-s2.0-85046462895

Number Of Pages


  • 8

Start Page


  • 2691

End Page


  • 2699

Volume


  • 19

Issue


  • 7

Place Of Publication


  • United States

Abstract


  • We present a star copolypeptide-based hydrogel ink capable of structural microfabrication using 3D extrusion printing. The material comprises an amphiphilic block copolymer structure of poly(benzyl-l-glutamate)-b-oligo(l-valine), which spontaneously forms hydrogels through hydrophobic interactions. The chemical design allows the bulk phase of the hydrogel to remain intact after application of shear due to its self-recovery behavior. It is demonstrated that the composition of the materials is ideally suited for 3D printing with scaffolds capable of maintaining structural cohesion after extrusion. Post extrusion UV-triggered fixation of the printed structures is carried out, resulting in stable hydrogel constructs. The constructs were found to be degradable, exhibited favorable release of encapsulated molecular cargo, and do not appear to affect the metabolic health of the commonly used fibroblastic cell line Balb/3T3 in the absence of the reactive diluent N,N′-methylenebis(acrylamide). The star copolypeptide inks allow for rapid prototyping enabling the fabrication of defined intricate microstructures, providing a platform for complex scaffold development that would otherwise be unattainable with other processing techniques such as molding or casting.

Authors


  •   Murphy, Robert (external author)
  •   Walsh, David (external author)
  •   Hamilton, Charles (external author)
  •   Cryan, Sally-Ann (external author)
  •   in het Panhuis, Marc
  •   Heise, Andreas (external author)

Publication Date


  • 2018

Citation


  • Murphy, R., Walsh, D. P., Hamilton, C. A., Cryan, S., in het Panhuis, M. & Heise, A. (2018). Degradable 3D-Printed Hydrogels Based on Star-Shaped Copolypeptides. Biomacromolecules, 19 (7), 2691-2699.

Scopus Eid


  • 2-s2.0-85046462895

Number Of Pages


  • 8

Start Page


  • 2691

End Page


  • 2699

Volume


  • 19

Issue


  • 7

Place Of Publication


  • United States