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Design of DNA rolling-circle templates with controlled fork topology to study mechanisms of DNA replication

Journal Article


Abstract


  • Rolling-circle DNA amplification is a powerful tool employed in biotechnology to produce large from small amounts of DNA. This mode of DNA replication proceeds via a DNA topology that resembles a replication fork, thus also providing experimental access to the molecular mechanisms of DNA replication. However, conventional templates do not allow controlled access to multiple fork topologies, which is an important factor in mechanistic studies. Here we present the design and production of a rolling-circle substrate with a tunable length of both the gap and the overhang, and we show its application to the bacterial DNA-replication reaction.

Publication Date


  • 2018

Citation


  • Monachino, E., Ghodke, H., Spinks, R. R., Hoatson, B. S., Jergic, S., Xu, Z., Dixon, N. E. & van Oijen, A. M. (2018). Design of DNA rolling-circle templates with controlled fork topology to study mechanisms of DNA replication. Analytical Biochemistry, 557 42-45.

Scopus Eid


  • 2-s2.0-85049984855

Number Of Pages


  • 3

Start Page


  • 42

End Page


  • 45

Volume


  • 557

Place Of Publication


  • United States

Abstract


  • Rolling-circle DNA amplification is a powerful tool employed in biotechnology to produce large from small amounts of DNA. This mode of DNA replication proceeds via a DNA topology that resembles a replication fork, thus also providing experimental access to the molecular mechanisms of DNA replication. However, conventional templates do not allow controlled access to multiple fork topologies, which is an important factor in mechanistic studies. Here we present the design and production of a rolling-circle substrate with a tunable length of both the gap and the overhang, and we show its application to the bacterial DNA-replication reaction.

Publication Date


  • 2018

Citation


  • Monachino, E., Ghodke, H., Spinks, R. R., Hoatson, B. S., Jergic, S., Xu, Z., Dixon, N. E. & van Oijen, A. M. (2018). Design of DNA rolling-circle templates with controlled fork topology to study mechanisms of DNA replication. Analytical Biochemistry, 557 42-45.

Scopus Eid


  • 2-s2.0-85049984855

Number Of Pages


  • 3

Start Page


  • 42

End Page


  • 45

Volume


  • 557

Place Of Publication


  • United States