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Using yeast hybrid system to identify proteins binding to small molecules

Chapter


Abstract


  • Protein–small molecule interaction studies provide useful insights into biological processes taking place within the living cell. A special yeast hybrid system, the yeast three-hybrid method, has been developed and used to explore proteins that bind to small molecules, by which means it may be possible to unravel biological processes and dissect function of biological systems. Here we present a protocol employing this method for identifying such binding proteins.

Authors


  •   Wang, You (external author)
  •   Letham, David S. (external author)
  •   John, Peter C. L. (external author)
  •   Zhang, Ren

Publication Date


  • 2018

Citation


  • Wang, Y., Letham, D. S., John, P. C. L. & Zhang, R. (2018). Using yeast hybrid system to identify proteins binding to small molecules. In L. Onate-Sanchez (Ed.), Two-Hybrid Systems: Methods and Protocols (pp. 225-234). New York: Humana Press.

International Standard Book Number (isbn) 13


  • 9781493978717

Scopus Eid


  • 2-s2.0-85048055263

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers1/181

Book Title


  • Two-Hybrid Systems: Methods and Protocols

Start Page


  • 225

End Page


  • 234

Place Of Publication


  • New York

Abstract


  • Protein–small molecule interaction studies provide useful insights into biological processes taking place within the living cell. A special yeast hybrid system, the yeast three-hybrid method, has been developed and used to explore proteins that bind to small molecules, by which means it may be possible to unravel biological processes and dissect function of biological systems. Here we present a protocol employing this method for identifying such binding proteins.

Authors


  •   Wang, You (external author)
  •   Letham, David S. (external author)
  •   John, Peter C. L. (external author)
  •   Zhang, Ren

Publication Date


  • 2018

Citation


  • Wang, Y., Letham, D. S., John, P. C. L. & Zhang, R. (2018). Using yeast hybrid system to identify proteins binding to small molecules. In L. Onate-Sanchez (Ed.), Two-Hybrid Systems: Methods and Protocols (pp. 225-234). New York: Humana Press.

International Standard Book Number (isbn) 13


  • 9781493978717

Scopus Eid


  • 2-s2.0-85048055263

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers1/181

Book Title


  • Two-Hybrid Systems: Methods and Protocols

Start Page


  • 225

End Page


  • 234

Place Of Publication


  • New York