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High Throughput Cell-Free Extraction of Plasma by an Integrated Microfluidic Device Combining Inertial Focusing and Membrane

Journal Article


Abstract


  • Plasma is a host of numerous analytes such as proteins, metabolites, circulating nucleic

    acids (CNAs), and pathogens, and it contains massive information about the functioning

    of the whole body, which is of great importance for the clinical diagnosis. Plasma needs

    to be completely cell-free for effective detection of these analytes. The key process of

    plasma extraction is to eliminate the contamination from blood cells. Centrifugation, a

    golden standard method for blood separation, is generally lab-intensive, time consuming,

    and even dangerous to some extent, and needs to be operated by well-trained staffs.

    Membrane filtration can filter cells very effectively according to its pore size, but it is

    prone to clogging by dense particle concentration and suffers from limited capacity of filtration.

    Frequent rinse is lab-intensive and undesirable. In this work, we proposed and

    fabricated an integrated microfluidic device that combined particle inertial focusing and

    membrane filter for high efficient blood plasma separation. The integrated microfluidic

    device was evaluated by the diluted (1/10, 1/20) whole blood, and the quality of the

    extracted blood plasma was measured and compared with that from the standard centrifugation.

    We found that the quality of the extracted blood plasma from the proposed

    device can be equivalent to that from the standard centrifugation. This study demonstrates

    a significant progress toward the practical application of inertial microfluidics

    with membrane filter for high-throughput and highly efficient blood plasma extraction.

Authors


  •   Yuan, Dan (external author)
  •   Zhang, Jun (external author)
  •   Yan, Sheng (external author)
  •   Alici, Gursel
  •   Nguyen, Nam-Trung (external author)
  •   Li, Weihua

Publication Date


  • 2017

Citation


  • Zhang, J., Yan, S., Yuan, D., Alici, G., Nguyen, N. & Li, W. (2017). High Throughput Cell-Free Extraction of Plasma by an Integrated Microfluidic Device Combining Inertial Focusing and Membrane. Journal Of Heat Transfer-transactions Of The Asme, 139 (5), 052404-1-052404-7.

Scopus Eid


  • 2-s2.0-85027274933

Start Page


  • 052404-1

End Page


  • 052404-7

Volume


  • 139

Issue


  • 5

Place Of Publication


  • United States

Abstract


  • Plasma is a host of numerous analytes such as proteins, metabolites, circulating nucleic

    acids (CNAs), and pathogens, and it contains massive information about the functioning

    of the whole body, which is of great importance for the clinical diagnosis. Plasma needs

    to be completely cell-free for effective detection of these analytes. The key process of

    plasma extraction is to eliminate the contamination from blood cells. Centrifugation, a

    golden standard method for blood separation, is generally lab-intensive, time consuming,

    and even dangerous to some extent, and needs to be operated by well-trained staffs.

    Membrane filtration can filter cells very effectively according to its pore size, but it is

    prone to clogging by dense particle concentration and suffers from limited capacity of filtration.

    Frequent rinse is lab-intensive and undesirable. In this work, we proposed and

    fabricated an integrated microfluidic device that combined particle inertial focusing and

    membrane filter for high efficient blood plasma separation. The integrated microfluidic

    device was evaluated by the diluted (1/10, 1/20) whole blood, and the quality of the

    extracted blood plasma was measured and compared with that from the standard centrifugation.

    We found that the quality of the extracted blood plasma from the proposed

    device can be equivalent to that from the standard centrifugation. This study demonstrates

    a significant progress toward the practical application of inertial microfluidics

    with membrane filter for high-throughput and highly efficient blood plasma extraction.

Authors


  •   Yuan, Dan (external author)
  •   Zhang, Jun (external author)
  •   Yan, Sheng (external author)
  •   Alici, Gursel
  •   Nguyen, Nam-Trung (external author)
  •   Li, Weihua

Publication Date


  • 2017

Citation


  • Zhang, J., Yan, S., Yuan, D., Alici, G., Nguyen, N. & Li, W. (2017). High Throughput Cell-Free Extraction of Plasma by an Integrated Microfluidic Device Combining Inertial Focusing and Membrane. Journal Of Heat Transfer-transactions Of The Asme, 139 (5), 052404-1-052404-7.

Scopus Eid


  • 2-s2.0-85027274933

Start Page


  • 052404-1

End Page


  • 052404-7

Volume


  • 139

Issue


  • 5

Place Of Publication


  • United States