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Single-particle fusion of influenza viruses reveals complex interactions with target membranes

Journal Article


Abstract


  • The first step in infection of influenza A virus is contact with the host cell membrane, with which it later fuses. The composition of the target bilayer exerts a complex influence on both fusion efficiency and time. Here, an in vitro, single-particle approach is used to study this effect. Using total internal reflection fluorescence (TIRF) microscopy and a microfluidic flow cell, the hemifusion of single virions is visualized. Hemifusion efficiency and kinetics are studied while altering target bilayer cholesterol content and sialic-acid donor. Cholesterol ratios tested were 0%, 10%, 20%, and 40%. Sialic-acid donors GD1a and GYPA were used. Both cholesterol ratio and sialic-acid donors proved to have a significant effect on hemifusion efficiency. Furthermore, comparison between GD1a and GYPA conditions shows that the cholesterol dependence of the hemifusion time is severely affected by the sialic-acid donor. Only GD1a shows a clear increasing trend in hemifusion efficiency and time with increasing cholesterol concentration of the target bilayer with maximum rates for GD1A and 40% cholesterol. Overall our results show that sialic acid donor and target bilayer composition should be carefully chosen, depending on the desired hemifusion time and efficiency in the experiment.

Authors


  •   Van der borg, Guus (external author)
  •   Braddock, Scarlett (external author)
  •   Blijleven, Jelle S. (external author)
  •   van Oijen, Antoine M.
  •   Roos, Wouter (external author)

Publication Date


  • 2018

Citation


  • van der borg, G., Braddock, S., Blijleven, J. S., van Oijen, A. M. & Roos, W. H. (2018). Single-particle fusion of influenza viruses reveals complex interactions with target membranes. Journal of Physics Condensed Matter, 30 (20), 204005-1-204005-7.

Scopus Eid


  • 2-s2.0-85046273403

Start Page


  • 204005-1

End Page


  • 204005-7

Volume


  • 30

Issue


  • 20

Place Of Publication


  • United Kingdom

Abstract


  • The first step in infection of influenza A virus is contact with the host cell membrane, with which it later fuses. The composition of the target bilayer exerts a complex influence on both fusion efficiency and time. Here, an in vitro, single-particle approach is used to study this effect. Using total internal reflection fluorescence (TIRF) microscopy and a microfluidic flow cell, the hemifusion of single virions is visualized. Hemifusion efficiency and kinetics are studied while altering target bilayer cholesterol content and sialic-acid donor. Cholesterol ratios tested were 0%, 10%, 20%, and 40%. Sialic-acid donors GD1a and GYPA were used. Both cholesterol ratio and sialic-acid donors proved to have a significant effect on hemifusion efficiency. Furthermore, comparison between GD1a and GYPA conditions shows that the cholesterol dependence of the hemifusion time is severely affected by the sialic-acid donor. Only GD1a shows a clear increasing trend in hemifusion efficiency and time with increasing cholesterol concentration of the target bilayer with maximum rates for GD1A and 40% cholesterol. Overall our results show that sialic acid donor and target bilayer composition should be carefully chosen, depending on the desired hemifusion time and efficiency in the experiment.

Authors


  •   Van der borg, Guus (external author)
  •   Braddock, Scarlett (external author)
  •   Blijleven, Jelle S. (external author)
  •   van Oijen, Antoine M.
  •   Roos, Wouter (external author)

Publication Date


  • 2018

Citation


  • van der borg, G., Braddock, S., Blijleven, J. S., van Oijen, A. M. & Roos, W. H. (2018). Single-particle fusion of influenza viruses reveals complex interactions with target membranes. Journal of Physics Condensed Matter, 30 (20), 204005-1-204005-7.

Scopus Eid


  • 2-s2.0-85046273403

Start Page


  • 204005-1

End Page


  • 204005-7

Volume


  • 30

Issue


  • 20

Place Of Publication


  • United Kingdom