Skip to main content
placeholder image

Generation of Neural Organoids from Human Embryonic Stem Cells Using the Rotary Cell Culture System: Effects of Microgravity on Neural Progenitor Cell Fate

Journal Article


Abstract


  • Progress in aeronautics and spaceflight technologies requires in parallel further research on how microgravity may affect human tissue. To date, little is known about the effects of microgravity on human development. In this study we used the rotary cell culture system to investigate whether microgravity supports the generation and maintenance of neural organoids derived from human embryonic stem cells (hESCs) as a model of human brain development. Our results show that although neural organoids could be generated and maintained in microgravity conditions, there were changes in expression of rostral–caudal neural patterning genes and cortical markers compared to organoids generated in standard conditions. This phenomenon was also observed in hESC-derived cortical organoids exposed to microgravity for relatively shorter periods. These results are one of the first for analyzing human neurogenesis in a microgravity environment.

Authors


  •   Mattei, Cristiana (external author)
  •   Alshawaf, Abdullah (external author)
  •   D'Abaco, Giovanna (external author)
  •   Nayagam, Bryony A. (external author)
  •   Dottori, Mirella

Publication Date


  • 2018

Citation


  • Mattei, C., Alshawaf, A., D'Abaco, G., Nayagam, B. & Dottori, M. (2018). Generation of Neural Organoids from Human Embryonic Stem Cells Using the Rotary Cell Culture System: Effects of Microgravity on Neural Progenitor Cell Fate. Stem Cells And Development, 27 (12), 848-857.

Scopus Eid


  • 2-s2.0-85048377338

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1235

Number Of Pages


  • 9

Start Page


  • 848

End Page


  • 857

Volume


  • 27

Issue


  • 12

Place Of Publication


  • United States

Abstract


  • Progress in aeronautics and spaceflight technologies requires in parallel further research on how microgravity may affect human tissue. To date, little is known about the effects of microgravity on human development. In this study we used the rotary cell culture system to investigate whether microgravity supports the generation and maintenance of neural organoids derived from human embryonic stem cells (hESCs) as a model of human brain development. Our results show that although neural organoids could be generated and maintained in microgravity conditions, there were changes in expression of rostral–caudal neural patterning genes and cortical markers compared to organoids generated in standard conditions. This phenomenon was also observed in hESC-derived cortical organoids exposed to microgravity for relatively shorter periods. These results are one of the first for analyzing human neurogenesis in a microgravity environment.

Authors


  •   Mattei, Cristiana (external author)
  •   Alshawaf, Abdullah (external author)
  •   D'Abaco, Giovanna (external author)
  •   Nayagam, Bryony A. (external author)
  •   Dottori, Mirella

Publication Date


  • 2018

Citation


  • Mattei, C., Alshawaf, A., D'Abaco, G., Nayagam, B. & Dottori, M. (2018). Generation of Neural Organoids from Human Embryonic Stem Cells Using the Rotary Cell Culture System: Effects of Microgravity on Neural Progenitor Cell Fate. Stem Cells And Development, 27 (12), 848-857.

Scopus Eid


  • 2-s2.0-85048377338

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1235

Number Of Pages


  • 9

Start Page


  • 848

End Page


  • 857

Volume


  • 27

Issue


  • 12

Place Of Publication


  • United States