Skip to main content
placeholder image

Generation of RAB39B knockout isogenic human embryonic stem cell lines to model RAB39B-mediated Parkinson's disease

Journal Article


Download full-text (Open Access)

Abstract


  • Mutations in RAB39B are a known cause of X-linked early onset Parkinson's disease. Isogenic human embryonic stem cell lines carrying two independent deletions of RAB39B were generated using CRISPR/Cas9 genome editing tool. The deletions were confirmed by PCR and direct sequence analysis in two edited stem cell lines. Both cell lines showed pluripotency and displayed a normal karyotype. Further, they were able to form embryoid bodies in vitro, and express markers indicative of differentiation to the three germ layers.

Authors


  •   Gao, Yujing (external author)
  •   Wilson, Gabrielle (external author)
  •   Bozaoglu, Kiymet (external author)
  •   Elefanty, Andrew (external author)
  •   Stanley, Edouard (external author)
  •   Dottori, Mirella
  •   Lockhart, Paul J. (external author)

Publication Date


  • 2018

Citation


  • Gao, Y., Wilson, G., Bozaoglu, K., Elefanty, A. G., Stanley, E. G., Dottori, M. & Lockhart, P. J. (2018). Generation of RAB39B knockout isogenic human embryonic stem cell lines to model RAB39B-mediated Parkinson's disease. Stem Cell Research, 28 161-164.

Scopus Eid


  • 2-s2.0-85042468424

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2234&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1207

Number Of Pages


  • 3

Start Page


  • 161

End Page


  • 164

Volume


  • 28

Place Of Publication


  • Netherlands

Abstract


  • Mutations in RAB39B are a known cause of X-linked early onset Parkinson's disease. Isogenic human embryonic stem cell lines carrying two independent deletions of RAB39B were generated using CRISPR/Cas9 genome editing tool. The deletions were confirmed by PCR and direct sequence analysis in two edited stem cell lines. Both cell lines showed pluripotency and displayed a normal karyotype. Further, they were able to form embryoid bodies in vitro, and express markers indicative of differentiation to the three germ layers.

Authors


  •   Gao, Yujing (external author)
  •   Wilson, Gabrielle (external author)
  •   Bozaoglu, Kiymet (external author)
  •   Elefanty, Andrew (external author)
  •   Stanley, Edouard (external author)
  •   Dottori, Mirella
  •   Lockhart, Paul J. (external author)

Publication Date


  • 2018

Citation


  • Gao, Y., Wilson, G., Bozaoglu, K., Elefanty, A. G., Stanley, E. G., Dottori, M. & Lockhart, P. J. (2018). Generation of RAB39B knockout isogenic human embryonic stem cell lines to model RAB39B-mediated Parkinson's disease. Stem Cell Research, 28 161-164.

Scopus Eid


  • 2-s2.0-85042468424

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2234&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1207

Number Of Pages


  • 3

Start Page


  • 161

End Page


  • 164

Volume


  • 28

Place Of Publication


  • Netherlands